Back to Search
Start Over
Phosphate Solubilization and Gene Expression of Phosphate-Solubilizing Bacterium Burkholderia multivorans WS-FJ9 under Different Levels of Soluble Phosphate.
- Source :
-
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2017 Apr 28; Vol. 27 (4), pp. 844-855. - Publication Year :
- 2017
-
Abstract
- Phosphate-solubilizing bacteria (PSB) have the ability to dissolve insoluble phosphate and enhance soil fertility. However, the growth and mineral phosphate solubilization of PSB could be affected by exogenous soluble phosphate and the mechanism has not been fully understood. In the present study, the growth and mineral phosphate-solubilizing characteristics of PSB strain Burkholderia multivorans WS-FJ9 were investigated at six levels of exogenous soluble phosphate (0, 0.5, 1, 5, 10, and 20 mM). The WS-FJ9 strain showed better growth at high levels of soluble phosphate. The phosphate-solubilizing activity of WS-FJ9 was reduced as the soluble phosphate concentration increased, as well as the production of pyruvic acid. Transcriptome profiling of WS-FJ9 at three levels of exogenous soluble phosphate (0, 5, and 20 mM) identified 446 differentially expressed genes, among which 44 genes were continuously up-regulated when soluble phosphate concentration was increased and 81 genes were continuously down-regulated. Some genes related to cell growth were continuously up-regulated, which would account for the better growth of WS-FJ9 at high levels of soluble phosphate. Genes involved in glucose metabolism, including glycerate kinase, 2-oxoglutarate dehydrogenase, and sugar ABC-type transporter, were continuously down-regulated, which indicates that metabolic channeling of glucose towards the phosphorylative pathway was negatively regulated by soluble phosphate. These findings represent an important first step in understanding the molecular mechanisms of soluble phosphate effects on the growth and mineral phosphate solubilization of PSB.
- Subjects :
- Bacterial Proteins biosynthesis
Bacterial Proteins drug effects
Bacterial Proteins genetics
Burkholderia enzymology
Burkholderia growth & development
Culture Media chemistry
DNA, Bacterial
Gene Expression Profiling methods
Gene Expression Regulation, Bacterial drug effects
Gene Ontology
Genes, Bacterial genetics
Glucose metabolism
Hydrogen-Ion Concentration
Ketoglutarate Dehydrogenase Complex biosynthesis
Ketoglutarate Dehydrogenase Complex drug effects
Ketoglutarate Dehydrogenase Complex genetics
Metabolic Networks and Pathways drug effects
Metabolic Networks and Pathways genetics
Molecular Sequence Annotation
Phosphates administration & dosage
Phosphotransferases (Alcohol Group Acceptor) biosynthesis
Phosphotransferases (Alcohol Group Acceptor) drug effects
Phosphotransferases (Alcohol Group Acceptor) genetics
Pyruvic Acid metabolism
RNA, Bacterial isolation & purification
Real-Time Polymerase Chain Reaction methods
Soil
Solubility
Transcriptome genetics
Up-Regulation
Burkholderia genetics
Burkholderia metabolism
Gene Expression Regulation, Bacterial genetics
Phosphates chemistry
Phosphates metabolism
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1738-8872
- Volume :
- 27
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 28138122
- Full Text :
- https://doi.org/10.4014/jmb.1611.11057