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Near Infrared Photoimmunotherapy in a Transgenic Mouse Model of Spontaneous Epidermal Growth Factor Receptor (EGFR)-expressing Lung Cancer.
- Source :
-
Molecular cancer therapeutics [Mol Cancer Ther] 2017 Feb; Vol. 16 (2), pp. 408-414. Date of Electronic Publication: 2016 Nov 15. - Publication Year :
- 2017
-
Abstract
- Near infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that combines the specificity of antibodies for targeting tumors with the toxicity induced by a sensitive photoabsorber following exposure to NIR light. Most studies of NIR-PIT have been performed in xenograft models of cancer. The purpose of this study was to evaluate the therapeutic effects of NIR-PIT in a transgenic model of spontaneous lung cancer expressing human EGFR (hEGFR-TL). Mice were separated into 3 groups for the following treatments: (1) no treatment (control); (2) 150 μg of photoabsorber, IR700, conjugated to panitumumab, an antibody targeting EGFR [antibody-photoabsorber conjugate (APC)] intravenously (i.v.) only; (3) 150 μg of APC i.v. with NIR light administration. Each treatment was performed every week up to three weeks. MRI was performed 1 day before and 3, 6, 13, 20, 27, and 34 days after first NIR-PIT. The relative volume of lung tumors was calculated from the tumor volume at each MRI time point divided by the initial volume. Steel test for multiple comparisons was used to compare the tumor volume ratio with that of control. Tumor volume ratio was inhibited significantly in the NIR-PIT group compared with control group (P < 0.01 at all time points). In conclusion, NIR-PIT effectively treated a spontaneous lung cancer in a hEGFR-TL transgenic mouse model. MRI successfully monitored the therapeutic effects of NIR-PIT. Mol Cancer Ther; 16(2); 408-14. ©2016 AACR.<br /> (©2016 American Association for Cancer Research.)
- Subjects :
- Animals
Antineoplastic Agents pharmacology
Cell Line, Tumor
Disease Models, Animal
ErbB Receptors metabolism
Humans
Image Processing, Computer-Assisted
Immunoconjugates pharmacology
Immunohistochemistry
Lung Neoplasms diagnosis
Lung Neoplasms therapy
Magnetic Resonance Imaging
Mice
Mice, Transgenic
Microscopy, Fluorescence
Photosensitizing Agents pharmacology
Treatment Outcome
Xenograft Model Antitumor Assays
ErbB Receptors genetics
Gene Expression
Immunotherapy methods
Lung Neoplasms genetics
Phototherapy methods
Subjects
Details
- Language :
- English
- ISSN :
- 1538-8514
- Volume :
- 16
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular cancer therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 28151706
- Full Text :
- https://doi.org/10.1158/1535-7163.MCT-16-0663