Back to Search
Start Over
Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2017 Apr; Vol. 443, pp. 33-44. Date of Electronic Publication: 2017 Feb 03. - Publication Year :
- 2017
-
Abstract
- Antibodies are widely considered to be a frequent primary and often mechanistic correlate of protection of approved vaccines; thus evaluating the antibody response is of critical importance in attempting to understand and predict the efficacy of novel vaccine candidates. Historically, antibody responses have been analyzed by determining the titer of the humoral response using measurements such as an ELISA, neutralization, or agglutination assays. In the simplest case, sufficiently high titers of antibody against vaccine antigen(s) are sufficient to predict protection. However, antibody titer provides only a partial measure of antibody function, which is dependent on both the variable region (Fv) to bind the antigen target, and the constant region (Fc) to elicit an effector response from the innate arm of the immune system. In the case of some diseases, such as HIV, for which an effective vaccine has proven elusive, antibody effector function has been shown to be an important driver of monoclonal antibody therapy outcomes, of viral control in infected patients, and of vaccine-mediated protection in preclinical and clinical studies. We sought to establish a platform for the evaluation of the Fc domain characteristics of antigen-specific antibodies present in polyclonal samples in order to better develop insights into Fc receptor-mediated antibody effector activity, more fully understand how antibody responses may differ in association with disease progression and between subject groups, and differentiate protective from non-protective responses. To this end we have developed a high throughput biophysical platform capable of simultaneously evaluating many dimensions of the antibody effector response.<br /> (Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- AIDS Vaccines immunology
Animals
Antibody Affinity
Antibody-Dependent Cell Cytotoxicity
Binding Sites, Antibody
Case-Control Studies
Disease Models, Animal
Flow Cytometry
HIV Antibodies blood
HIV Infections blood
HIV Infections immunology
Humans
Immunization
Immunoglobulin Fc Fragments blood
Macaca mulatta
Phagocytosis
Protein Binding
Receptors, IgG genetics
Receptors, IgG metabolism
Simian Acquired Immunodeficiency Syndrome blood
Simian Acquired Immunodeficiency Syndrome immunology
Simian Acquired Immunodeficiency Syndrome therapy
Simian Immunodeficiency Virus immunology
AIDS Vaccines administration & dosage
HIV immunology
HIV Antibodies immunology
HIV Infections therapy
High-Throughput Screening Assays methods
Immunity, Humoral drug effects
Immunoglobulin Fc Fragments immunology
Immunologic Techniques
Receptors, IgG immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7905
- Volume :
- 443
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 28163018
- Full Text :
- https://doi.org/10.1016/j.jim.2017.01.010