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Dose-dependent inhibition of uterine contractility by nitric oxide: A potential mechanism underlying persistent breeding-induced endometritis in the mare.
- Source :
-
Theriogenology [Theriogenology] 2017 Mar 01; Vol. 90, pp. 59-64. Date of Electronic Publication: 2016 Nov 28. - Publication Year :
- 2017
-
Abstract
- Nitric oxide (NO) may have a role in persistent breeding-induced endometritis in mares through an inhibitory effect on uterine contractility. The objectives of this study were to test the effect of NO on spontaneous uterine contractility in-vitro and to evaluate whether this effect varied between the longitudinal and circular muscle layers of the uterus. Reproductive tracts were collected from eight euthanized non-pregnant mares (age 4-19 years; body weight 405-530 kg). Transrectal examination of the reproductive tract was performed before euthanasia to evaluate stage of the estrous cycle and presence of any apparent abnormality. After euthanasia, one uterine tissue sample was collected for histological evaluation and four full-thickness uterine tissue strips (10-12 mm × 2 mm), two parallel to each muscle layer, were excised for in-vitro contractility evaluation. Strips were suspended in tissue chambers containing Krebs-Henseleit solution, with continuous aeration (95% O <subscript>2</subscript> -5% CO <subscript>2</subscript> ; pH 7.4) at 37 °C. After equilibration, spontaneous contractility was recorded (pre-treatment) and strips excised in each direction were randomly allocated to each of two groups: 1) SNAP (S-nitroso-N-acetylpenicillamine, an NO donor); or 2) NAP (N-acetyl-d-penicillamine, vehicle and time-matched control). These were treated at 15 min intervals with increasing concentrations (10 <superscript>-7</superscript>  M to 10 <superscript>-3</superscript>  M) of SNAP and NAP, respectively. Contractility data was recorded throughout the experiment. An interaction effect of group-by-concentration was observed (P < 0.0001). The mean contractility after treatment with 10 <superscript>-4</superscript>  M and 10 <superscript>-3</superscript>  M SNAP were significantly lower than the pre-treatment contractility and the mean contractility after treatment with lower SNAP concentrations. In contrast, contractility did not change significantly in the NAP treated controls. The effect of treatment on uterine contractility was not influenced by age or weight of the mare, stage of estrous cycle, uterine histology grade, or muscle layer. Secondary findings included significant main effects of stage of estrous cycle (increased contractility in estrus compared to diestrus), uterine histology grade (decreased contractility in grade IIB compared to grade I) and age (decreased contractility in mares aged > 8 years compared to mares aged ≤ 8 years). In conclusion, results of this study indicate that NO has a dose-dependent inhibitory effect on spontaneous uterine contractility irrespective of the muscle layer in the mare.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Dose-Response Relationship, Drug
Endometritis etiology
Endometritis physiopathology
Female
Horse Diseases etiology
Myometrium drug effects
Myometrium physiopathology
Penicillamine analogs & derivatives
Penicillamine pharmacology
Pregnancy
S-Nitroso-N-Acetylpenicillamine pharmacology
Uterus drug effects
Uterus physiopathology
Endometritis veterinary
Horse Diseases physiopathology
Horses physiology
Nitric Oxide metabolism
Nitric Oxide Donors pharmacology
Uterine Contraction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3231
- Volume :
- 90
- Database :
- MEDLINE
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 28166989
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2016.11.026