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The study of polyphenolic compounds profile of some Rosmarinus officinalis L. extracts.
- Source :
-
Pakistan journal of pharmaceutical sciences [Pak J Pharm Sci] 2016 Nov; Vol. 29 (6 Suppl), pp. 2355-2361. - Publication Year :
- 2016
-
Abstract
- The purpose of this study was to compare the polyphenol profile of different Rosemary (Rosmarinus officinalis) extracts obtained from dry and fresh herb and the evaluation of their antioxidant effect. There were studied the polyphenols from hydroalcoholic extracts (1:5- dry plant:solvent) obtained from fresh respectively dry plant and the gemmotherapic extract obtained from fresh plant (1:20- dry plant: solvent). The polyphenol profile was evaluated by UV-Vis spectrophotometry, TLC and HPLC. The total polyphenol respectively flavonoids content were determined by UV-V is spectrophotometry. The antioxidant effect was evaluated by DPPH, ABTS, FRAP, CUPRAC and silver nanoparticle (SNP) methods. The hydroalcoholic extract obtained from fresh plant contains the highest concentration of total polyphenols, (0.601mg/ml rosmarinic acid), total flavonoids, (0.270mg/ml luteoline) and rosmarinic acid (0.350 mg/ml). The less concentrated is the gemmotherapic extract, but also the extraction ratio is higher than the hydroalcoholic extracts. The high content in polyphenols of the mentioned hydroalcoholic extract was confirmed by highest values of antioxidant activity: 39.1ml (DPPH), 7.7 ml (ABTS), 698mM ET/100 ml (FRAP), 1947 mM ET/100 ml (CUPRAC), 4570mM ET/100 ml (SNP). These differences in the polyphenols profiles show the importance of use the fresh plants for obtaining the good quality extracts.
- Subjects :
- Antioxidants pharmacology
Benzothiazoles chemistry
Biphenyl Compounds chemistry
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Oxidation-Reduction
Oxygen Radical Absorbance Capacity
Phytotherapy
Picrates chemistry
Plant Leaves chemistry
Plant Shoots chemistry
Plants, Medicinal
Polyphenols pharmacology
Solvents chemistry
Spectrophotometry, Ultraviolet
Sulfonic Acids chemistry
Antioxidants analysis
Plant Extracts chemistry
Polyphenols analysis
Rosmarinus chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1011-601X
- Volume :
- 29
- Issue :
- 6 Suppl
- Database :
- MEDLINE
- Journal :
- Pakistan journal of pharmaceutical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28167478