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Involvement of the phosphoinositide 3-kinase/Akt signaling pathway in bone morphogenetic protein 9-stimulated osteogenic differentiation and stromal cell-derived factor 1 production in human periodontal ligament fibroblasts.
- Source :
-
European journal of oral sciences [Eur J Oral Sci] 2017 Apr; Vol. 125 (2), pp. 119-126. Date of Electronic Publication: 2017 Feb 13. - Publication Year :
- 2017
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Abstract
- Recent studies have shown that bone morphogenetic protein 9 (BMP-9) can induce osteogenic differentiation in human periodontal stem cells and human periodontal ligament fibroblasts (PDLFs). Bone morphogenetic protein 9 may be used in periodontal tissue regeneration because of its potent osteoinductive ability. Human periodontal ligament cells also have been demonstrated to produce stromal cell-derived factor 1 (SDF-1), which is important for stem-cell homing and recruitment to injured sites. In the present study, we examined the involvement of the phosphoinositide 3-kinase (PI3K)/Akt signaling axis in osteogenic differentiation and SDF-1 production in human PDLFs stimulated with BMP-9 in osteogenic medium supplemented with dexamethasone and ascorbic acid. Pretreatment of the cells with LY294002, a PI3K-specific inhibitor, suppressed not only BMP-9-enhanced alkaline phosphatase activity but also expression of a BMP-response gene (inhibitor of DNA binding 1) and osteogenic marker genes (runt-related transcription factor 2, osterix, bone sialoprotein, and osteopontin). In addition, BMP-9 up-regulated SDF-1 production, and the production of SDF-1 was suppressed by LY294002. The protein SDF-1-alpha was identified as a major isoform of SDF-1 that was regulated by BMP-9. Our data suggest involvement of the PI3K/Akt pathway in BMP-9-stimulated osteogenic differentiation and SDF-1 production in PDLFs cultured in osteogenic medium.<br /> (© 2017 Eur J Oral Sci.)
- Subjects :
- Alkaline Phosphatase metabolism
Blotting, Western
Cell Differentiation
Cell Proliferation
Cells, Cultured
Chromones pharmacology
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Morpholines pharmacology
Real-Time Polymerase Chain Reaction
Signal Transduction
Chemokine CXCL12 metabolism
Growth Differentiation Factor 2 pharmacology
Osteogenesis physiology
Periodontal Ligament cytology
Phosphatidylinositol 3-Kinases physiology
Proto-Oncogene Proteins c-akt physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0722
- Volume :
- 125
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- European journal of oral sciences
- Publication Type :
- Academic Journal
- Accession number :
- 28191670
- Full Text :
- https://doi.org/10.1111/eos.12336