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High FMNL3 expression promotes nasopharyngeal carcinoma cell metastasis: role in TGF-β1-induced epithelia-to-mesenchymal transition.
- Source :
-
Scientific reports [Sci Rep] 2017 Feb 15; Vol. 7, pp. 42507. Date of Electronic Publication: 2017 Feb 15. - Publication Year :
- 2017
-
Abstract
- Formin-like 3 (FMNL3) plays a crucial role in cytoskeletal mediation and is potentially a biomarker for cell migration; however, its role in cancer metastasis remains unknown. In this study, we found elevated FMNL3 protein expression in clinical nasopharyngeal carcinoma (NPC) tissues. FMNL3 expression positively correlated to the clinical stage, T (tumour), N (lymph node metastasis) and M (distant metastasis) classification of NPC patients. Moreover, FMNL3 positively correlated to Vimentin expression and negatively correlated to E-cadherin expression in clinical NPC samples. In vitro experiments showed that FMNL3 expression was inversely related to NPC cell differentiation status. Overexpression of FMNL3 led to epithelial-to-mesenchymal transition (EMT) in well differentiated CNE1 cells. TGF-β1-treated poorly differentiated CNE2 cells showed changes in EMT accompanied by enhanced FMNL3 expression and cell migration. On the contrary, knockdown of FMNL3 partially attenuated the TGF-β1-promoted CNE2 cell migration, together with associated changes in EMT markers. Finally, knockdown of FMNL3 also weakened EMT in tumours in xenographs. Our study indicates for the first time that TGF-β1/FMNL3 signalling may be a novel mechanism mediating EMT in NPC, which is closely associated with NPC metastasis.
- Subjects :
- Adult
Aged
Biomarkers
Cadherins metabolism
Carcinoma metabolism
Carcinoma pathology
Cell Line, Tumor
Cell Movement
Epithelial-Mesenchymal Transition drug effects
Epithelial-Mesenchymal Transition genetics
Female
Formins
Gene Expression
Humans
Immunohistochemistry
Male
Middle Aged
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms metabolism
Nasopharyngeal Neoplasms pathology
Proteins metabolism
Transforming Growth Factor beta1 metabolism
Vimentin metabolism
Carcinoma genetics
Nasopharyngeal Neoplasms genetics
Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28198387
- Full Text :
- https://doi.org/10.1038/srep42507