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Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer.
- Source :
-
International journal of cancer [Int J Cancer] 2017 May 15; Vol. 140 (10), pp. 2298-2309. Date of Electronic Publication: 2017 Mar 02. - Publication Year :
- 2017
-
Abstract
- Colorectal cancer is one of the major causes of death from cancer. Metastasis is the leading cause of treatment failure, in which cancer stem cells and circulating tumor cells play crucial roles. Identifying the involved metastatic biomarkers and clarifying the regulation mechanisms are of great importance for targeting tumor metastasis. In the current research, we discovered that KIAA1199, a cell-migration inducing protein, showed higher expression in CD44+ cancer cells from metastatic compared with the paired primary tissues, and was upregulated in colorectal cancer and positively correlated with numbers and mesenchymal phenotype of circulating tumor cells, and predicted shorter progress-free survival. Moreover, we indicated that down-regulation of KIAA1199 suppressed migration and invasion of colorectal cancer cells in vitro, and inhibited metastasis in vivo. Furthermore, we demonstrated that KIAA1199 was one of the direct and functional targets of miR-216a, and miR-216a overexpression led to decreased migration and invasion of colorectal cancer cells in vitro, and inhibited metastasis in vivo. Collectively, KIAA1199 plays a critical role in maintaining an aggressive phenotype of tumor cells, and suppression of KIAA1199-related motilities of tumor cells contributes to reduced tumor metastasis in colorectal cancer.<br /> (© 2017 UICC.)
- Subjects :
- Animals
Apoptosis
Biomarkers, Tumor genetics
Blotting, Western
Cell Movement
Cell Proliferation
Colorectal Neoplasms genetics
Colorectal Neoplasms metabolism
Epithelial-Mesenchymal Transition
Female
Fluorescent Antibody Technique
Humans
Hyaluronoglucosaminidase
Immunoenzyme Techniques
Liver Neoplasms genetics
Liver Neoplasms metabolism
Liver Neoplasms pathology
Male
Mice
Mice, Inbred NOD
Mice, SCID
MicroRNAs genetics
Middle Aged
Neoplasm Invasiveness
Neoplasm Staging
Neoplastic Stem Cells
Prognosis
Proteins genetics
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Survival Rate
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Biomarkers, Tumor metabolism
Colorectal Neoplasms pathology
Gene Expression Regulation, Neoplastic
Liver Neoplasms secondary
MicroRNAs metabolism
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0215
- Volume :
- 140
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 28213952
- Full Text :
- https://doi.org/10.1002/ijc.30656