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Neutrophil extracellular traps formation in patients with eosinophilic granulomatosis with polyangiitis: association with eosinophilic inflammation.
- Source :
-
Clinical and experimental rheumatology [Clin Exp Rheumatol] 2017 Mar-Apr; Vol. 35 Suppl 103 (1), pp. 27-32. Date of Electronic Publication: 2017 Feb 09. - Publication Year :
- 2017
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Abstract
- Objectives: Eosinophilic granulomatosis with polyangiitis (EGPA) is associated with an inflammation and the presence of antineutrophil cytoplasmic antibodies (ANCA). Thus, we investigated the impact of ANCAs and eosinophilic inflammation on neutrophil activation and extracellular traps (NETs) formation.<br />Methods: We recruited 29 patients in the remission of EGPA (17 ANCA-negative and 12 ANCA-positive, including 7 p-ANCA-positive and 5 c-ANCA-positive patients). Healthy donors' neutrophils were stimulated with EGPA patients' serum. NETs formation was assessed by immunofluorescence and scanning electron microscopy.<br />Results: EGPA patients presented enhanced ability to generate NETs compared to healthy subjects (20.3±8.2% vs. 2.7±1.5%, p=0.0036). However, there were no differences in NETs formation between ANCA-positive and ANCA-negative patients (23±11.2% vs. 17±6.1%, p=0.15). There was also no correlation between NETs generation and the amount of circulating DNA in EGPA patients. Among ANCA-positive patients, p-ANCA-positives showed the highest percentage of NETs as compared to cANCA-positive and ANCA-negative patients (27.3±10.3% vs. 17.8±10.5% and vs. 17±6.1%, both p<0.01, respectively). Eosinophils number correlated with the percentage of NETs in the whole EGPA group (r=0.53, p=0.039), but we failed to observe the correlation with an eosinophil cationic protein (r=0.49, p=0.058).<br />Conclusions: EGPA patients' serum has the ability to induce NETosis with no regard to the ANCA status in contrast to other vasculitides, where p-ANCA were considered as the main factor. Interestingly, NETs formation in EGPA patients connected with the number of eosinophils might be of major relevance. Further studies are required to assess which eosinophil-derived factors might be responsible for the neutrophils activation in EGPA patients.
- Subjects :
- Adult
Antibodies, Antineutrophil Cytoplasmic blood
Biomarkers blood
Case-Control Studies
Cells, Cultured
Churg-Strauss Syndrome diagnosis
Churg-Strauss Syndrome immunology
Eosinophil Cationic Protein blood
Eosinophils immunology
Extracellular Traps immunology
Female
Fluorescent Antibody Technique
Granulomatosis with Polyangiitis diagnosis
Granulomatosis with Polyangiitis immunology
Humans
Male
Microscopy, Electron, Scanning
Middle Aged
Neutrophils immunology
Neutrophils ultrastructure
Churg-Strauss Syndrome blood
Eosinophils metabolism
Extracellular Traps metabolism
Granulomatosis with Polyangiitis blood
Neutrophil Activation
Neutrophils metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0392-856X
- Volume :
- 35 Suppl 103
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Clinical and experimental rheumatology
- Publication Type :
- Academic Journal
- Accession number :
- 28229830