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Autocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin.
- Source :
-
Scientific reports [Sci Rep] 2017 Mar 02; Vol. 7, pp. 43564. Date of Electronic Publication: 2017 Mar 02. - Publication Year :
- 2017
-
Abstract
- Unusual intramolecular cross-links present in adhesins from Gram-positive bacteria have been used to develop a generic process amenable to biotechnology applications. Based on the crystal structure of RrgA, the Streptococcus pneumoniae pilus adhesin, we provide evidence that two engineered protein fragments retain their ability to associate covalently with high specificity, in vivo and in vitro, once isolated from the parent protein. We determined the optimal conditions for the assembly of the complex and we solved its crystal structure at 2 Å. Furthermore, we demonstrate biotechnological applications related to antibody production, nanoassembly and cell-surface labeling based on this process we named Bio Molecular Welding.
- Subjects :
- Adhesins, Bacterial genetics
Adhesins, Bacterial metabolism
Bacterial Proteins chemistry
Bacterial Proteins genetics
Bacterial Proteins metabolism
Catalysis
Fimbriae Proteins chemistry
Fimbriae Proteins genetics
Fimbriae Proteins metabolism
Models, Molecular
Molecular Weight
Multiprotein Complexes chemistry
Multiprotein Complexes metabolism
Protein Binding
Protein Conformation
Recombinant Fusion Proteins
Spectrometry, Mass, Electrospray Ionization
Virulence Factors chemistry
Virulence Factors genetics
Virulence Factors metabolism
Adhesins, Bacterial chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28252635
- Full Text :
- https://doi.org/10.1038/srep43564