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Effect of early pregnancy on the expression of progesterone receptor and progesterone-induced blocking factor in ovine lymph node.
- Source :
-
Theriogenology [Theriogenology] 2017 Apr 15; Vol. 93, pp. 78-83. Date of Electronic Publication: 2017 Feb 01. - Publication Year :
- 2017
-
Abstract
- Lymph nodes are the sites where the immune reaction or suppression takes place. Progesterone (P4) exerts an essential effect of the immunomodulation on the maternal uterus during early pregnancy in ruminants. At present study, the inguinal lymph nodes were obtained at day 16 of non-pregnancy, days 13, 16 and 25 of pregnancy (n = 3 for each group) in ewes, and RT-PCR assay, western blot and immunohistochemistry analysis were used to analyze to the effect of early pregnancy on the expression of P4 receptor (PGR) and progesterone-induced blocking factor (PIBF) in the lymph nodes. Our results showed that the PGR and PIBF mRNA were up-regulated in the lymph nodes in pregnant ewes, and the PGR isoform (60 kDa) and the PIBF variant (75 kDa) were expressed constantly in the lymph nodes. However, there was no expression of the PGR isoform (40 kDa) and the PIBF variant (48 kDa) at day 16 of the estrous cycle. The immunohistochemistry results confirmed that the PGR and PIBF proteins were limited to the subcapsular sinus and trabeculae in the cortex, medullary sinuses, and were localized in the cytoplasm of the specific cells. This paper reports for the first time that early pregnancy exerts its effect on the specific cells in the lymph nodes through P4, which results in the up-regulated expression of the PGR mRNA and 40 kDa isoform, the PIBF mRNA and 48 kDa variant, and is involved in the immunoregulation of the lymph nodes through a cytosolic pathway in ewes.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Female
Gene Expression drug effects
Gestational Age
Immunohistochemistry veterinary
Lymph Nodes immunology
Pregnancy
Pregnancy Proteins analysis
RNA, Messenger analysis
Receptors, Progesterone analysis
Suppressor Factors, Immunologic analysis
Lymph Nodes chemistry
Pregnancy Proteins genetics
Progesterone pharmacology
Receptors, Progesterone genetics
Sheep
Suppressor Factors, Immunologic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3231
- Volume :
- 93
- Database :
- MEDLINE
- Journal :
- Theriogenology
- Publication Type :
- Academic Journal
- Accession number :
- 28257871
- Full Text :
- https://doi.org/10.1016/j.theriogenology.2017.01.042