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Cytocompatibility of Wood-Derived Cellulose Nanofibril Hydrogels with Different Surface Chemistry.
- Source :
-
Biomacromolecules [Biomacromolecules] 2017 Apr 10; Vol. 18 (4), pp. 1238-1248. Date of Electronic Publication: 2017 Mar 16. - Publication Year :
- 2017
-
Abstract
- The current study aims to demonstrate the influence of the surface chemistry of wood-derived cellulose nanofibril (CNF) hydrogels on fibroblasts for tissue engineering applications. TEMPO-mediated oxidation or carboxymethylation pretreatments were employed to produce hydrogels with different surface chemistry. This study demonstrates the following: first, the gelation of CNF with cell culture medium and formation of stable hydrogels with improved rheological properties; second, the response of mouse fibroblasts cultured on the surface of the hydrogels or sandwiched within the materials with respect to cytotoxicity, cell attachment, proliferation, morphology, and migration. Indirect cytotoxicity tests showed no toxic effect of either hydrogel. The direct contact with the carboxymethylated hydrogel adversely influenced the morphology of the cells and limited their spreading, while typical morphology and spreading of cells were observed with the TEMPO-oxidized hydrogel. The porous fibrous structure may be a key to cell proliferation and migration in the hydrogels.
- Subjects :
- Animals
Cell Movement
Cell Proliferation
Cell Survival
Cells, Cultured
Fibroblasts chemistry
Fibroblasts cytology
Mice
Porosity
Rheology
Spectroscopy, Fourier Transform Infrared
Tissue Engineering
Biocompatible Materials chemistry
Cellulose chemistry
Hydrogels chemistry
Nanostructures chemistry
Wood chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1526-4602
- Volume :
- 18
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biomacromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 28263573
- Full Text :
- https://doi.org/10.1021/acs.biomac.6b01911