Back to Search
Start Over
O- Acyl Sugars Protect a Wild Tobacco from Both Native Fungal Pathogens and a Specialist Herbivore.
- Source :
-
Plant physiology [Plant Physiol] 2017 May; Vol. 174 (1), pp. 370-386. Date of Electronic Publication: 2017 Mar 08. - Publication Year :
- 2017
-
Abstract
- O -Acyl sugars ( O -AS) are abundant trichome-specific metabolites that function as indirect defenses against herbivores of the wild tobacco Nicotiana attenuata ; whether they also function as generalized direct defenses against herbivores and pathogens remains unknown. We characterized natural variation in O- AS among 26 accessions and examined their influence on two native fungal pathogens, Fusarium brachygibbosum U4 and Alternaria sp. U10, and the specialist herbivore Manduca sexta At least 15 different O- AS structures belonging to three classes were found in N. attenuata leaves. A 3-fold quantitative variation in total leaf O- AS was found among the natural accessions. Experiments with natural accessions and crosses between high- and low- O- AS accessions revealed that total O- AS levels were associated with resistance against herbivores and pathogens. Removing O- AS from the leaf surface increased M. sexta growth rate and plant fungal susceptibility. O -AS supplementation in artificial diets and germination medium reduced M. sexta growth and fungal spore germination, respectively. Finally, silencing the expression of a putative branched-chain α-ketoacid dehydrogenase E1 β-subunit-encoding gene ( NaBCKDE1B ) in the trichomes reduced total leaf O- AS by 20% to 30% and increased susceptibility to Fusarium pathogens. We conclude that O- AS function as direct defenses to protect plants from attack by both native pathogenic fungi and a specialist herbivore and infer that their diversification is likely shaped by the functional interactions among these biotic stresses.<br /> (© 2017 American Society of Plant Biologists. All Rights Reserved.)
- Subjects :
- 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) genetics
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) metabolism
Acylation
Alternaria physiology
Animals
Fusarium physiology
Gene Silencing
Herbivory physiology
Manduca physiology
Molecular Structure
Plant Diseases genetics
Plant Diseases microbiology
Plant Diseases parasitology
Plant Leaves microbiology
Plant Leaves parasitology
Plant Proteins genetics
Plant Proteins metabolism
Protein Subunits genetics
Protein Subunits metabolism
Nicotiana microbiology
Nicotiana parasitology
Trichomes genetics
Trichomes microbiology
Trichomes parasitology
Disease Resistance
Plant Leaves chemistry
Sugars chemistry
Nicotiana chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 174
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 28275149
- Full Text :
- https://doi.org/10.1104/pp.16.01904