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[Knockdown of Grb7 inhibits growth of oral squamous cell carcinoma, cell proliferation and promoted apoptosis through ERK/FOXM1 pathway].
- Source :
-
Shanghai kou qiang yi xue = Shanghai journal of stomatology [Shanghai Kou Qiang Yi Xue] 2016 Dec; Vol. 25 (6), pp. 688-693. - Publication Year :
- 2016
-
Abstract
- Purpose: To investigate the effect of growth factor receptor-bound 7 (Grb7) on oral squamous cell carcinoma growth and tumor xenografts.<br />Methods: Cal27 and hNOK cells were cultivated, real-time PCR and Western blotting were used to detect the expression of Grb7 in hNOK and Cal27. Cal27 was transfected with Grb7 siRNA for 48 h, cell proliferation was assayed using MTT. Flow cytometry was used to determine the cell cycle and apoptosis. Western blot was used to evaluate the expression of caspase3, Bax, bcl-2, Cyclin D1, Rb, E2F1, ERK and FOXM1. Grb7 siRNA and negative control were designed and injected subcutaneously into the mice, tumor volume and weight were measured. Statistical analysis was performed using SPSS 11.0 software package.<br />Results: Grb7 was highly expressed in Cal27 compared with hNOK. Depletion of Grb7 significantly inhibited cell proliferation, blocked G1/S phase transition, promoted cell apoptosis. Knockdown of Grb7 suppressed the expression of Cyclin D1 and Rb, upregulated E2F1 expression. Moreover, c-caspase 3 and Bax was also reduced after inhibition of Grb7. ERK/FOXM1 signaling pathway was also inhibited by Grb7. In addition, the volume and weight of tumor xenografts were reduced by siGrb7.<br />Conclusions: Depletion of Grb7 inhibits cell proliferation, promotes apoptosis and reduces tumor xenografts through ERK/FOXM1 pathway.
- Subjects :
- Animals
Apoptosis Regulatory Proteins
Caspase 3
Cell Cycle
Cyclin D1
GRB7 Adaptor Protein genetics
Gene Knockdown Techniques
Humans
MAP Kinase Signaling System
Mice
RNA, Small Interfering
Real-Time Polymerase Chain Reaction
Signal Transduction
Apoptosis
Carcinoma, Squamous Cell metabolism
Cell Line, Tumor
Cell Proliferation physiology
GRB7 Adaptor Protein metabolism
Mouth Neoplasms metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1006-7248
- Volume :
- 25
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Shanghai kou qiang yi xue = Shanghai journal of stomatology
- Publication Type :
- Academic Journal
- Accession number :
- 28275791