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Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa.
- Source :
-
Scientific reports [Sci Rep] 2017 Mar 09; Vol. 7, pp. 42392. Date of Electronic Publication: 2017 Mar 09. - Publication Year :
- 2017
-
Abstract
- Mammalian spermatozoa deliver various classes of RNAs to the oocyte during fertilization, and many of them may regulate fertility. The objective of the present study was to determine the composition and abundance of spermatozoal transcripts in fresh bull semen. The entire transcriptome of the spermatozoa from bulls (n = 3) was sequenced using two different platforms (Ion Proton and Illumina) to identify the maximum number of genes present in the spermatozoa. The bovine spermatozoa contained transcripts for 13,833 genes (transcripts per million, TPM > 10). Both intact and fragmented transcripts were found. These spermatozoal transcripts were associated with various stages of spermatogenesis, spermatozoal function, fertilization, and embryo development. The presence of intact transcripts of pregnancy-associated glycoproteins (PAGs) in the spermatozoa suggest a possible influence of sperm transcripts beyond early embryonic development. The specific regions (exon, intron, and exon-intron) of the particular spermatozoal transcripts might help regulate fertilization. This study demonstrates that the use of two different RNA-seq platforms provides a comprehensive profile of bovine spermatozoal RNA. Spermatozoal RNA profiling may be useful as a non-invasive method to delineate possible causes of male infertility and to predict fertility in a manner that is more effective than the conventional methods.
- Subjects :
- Animals
Aspartic Acid Endopeptidases metabolism
Cattle
Gene Expression Regulation
Gene Library
Male
Molecular Sequence Annotation
Pregnancy Proteins metabolism
RNA genetics
RNA isolation & purification
RNA metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Reproducibility of Results
Spermatozoa metabolism
Transcriptome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28276431
- Full Text :
- https://doi.org/10.1038/srep42392