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Glycine post-synthetic modification of MIL-53(Fe) metal-organic framework with enhanced and stable peroxidase-like activity for sensitive glucose biosensing.
- Source :
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Talanta [Talanta] 2017 May 15; Vol. 167, pp. 359-366. Date of Electronic Publication: 2017 Feb 20. - Publication Year :
- 2017
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Abstract
- A facile and rapid post-synthetic strategy was proposed to prepare a glycine functionalized MIL-53(Fe), namely glycine-MIL-53(Fe), by a simple mixing of water dispersible MIL-53(Fe) and glycine. The FT-IR, SEM, XRD and zeta potential were used to characterize the glycine-MIL-53(Fe). The result showed that glycine post-synthetic modification of MIL-53(Fe) did not change in the morphology and crystal structure of MIL-53(Fe). Interestingly, compared with MIL-53(Fe), the glycine-MIL-53(Fe) exhibits an enhanced peroxidase-like activity, which could catalyze the oxidation of TMB by H <subscript>2</subscript> O <subscript>2</subscript> to produce an intensive color reaction. Kinetic analysis indicated that the K <subscript>m</subscript> of glycine-MIL-53(Fe) for TMB was one-tenth of that of MIL-53(Fe). The glycine-MIL-53(Fe) as peroxidase mimetic displays better stability under alkaline or acidic conditions than MIL-53(Fe). The good performance of glycine-MIL-53(Fe) over MIL-53(Fe) may be attributed to the increase of affinity between TMB and the glycine-MIL-53(Fe). With these characteristics, a simple and sensitive method was developed for the detection of H <subscript>2</subscript> O <subscript>2</subscript> and glucose. The linear detection range for H <subscript>2</subscript> O <subscript>2</subscript> is 0.10-10μM with a detection limit of 49nM, and glucose could be linearly detected in the range from 0.25 to 10μM with a detection limit of 0.13μM. The proposed method was successfully used for glucose detection in human serum samples.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 167
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 28340732
- Full Text :
- https://doi.org/10.1016/j.talanta.2017.02.039