Back to Search
Start Over
DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture.
- Source :
-
Journal of immunology research [J Immunol Res] 2017; Vol. 2017, pp. 5252840. Date of Electronic Publication: 2017 Feb 27. - Publication Year :
- 2017
-
Abstract
- Abnormal immune response of the body against substances and tissues causes autoimmune diseases, such as polymyositis, dermatomyositis, and rheumatoid arthritis. Irregular lipid metabolism and inflammation may be a significant cause of autoimmune diseases. Although much progress has been made, mechanisms of initiation and proceeding of metabolic and inflammatory regulation in autoimmune disease have not been well-defined. And novel markers for the detection and therapy of autoimmune disease are urgent. mTOR signaling is a central regulator of extracellular metabolic and inflammatory processes, while DEP domain-containing mTOR-interacting protein (DEPTOR) is a natural inhibitor of mTOR. Here, we report that overexpression of DEPTOR reduces mTORC1 activity in lymphocytes of human peripheral blood mononuclear cells (PBMCs). Combination of DEPTOR overexpression and mTORC2/AKT inhibitors effectively inhibits lipogenesis and inflammation in lymphocytes of PBMC culture. Moreover, DEPTOR knockdown activates mTORC1 and increases lipogenesis and inflammations. Our findings provide a deep insight into the relationship between lipid metabolism and inflammations via DEPTOR-mTOR pathway and imply that DEPTOR-mTOR in lymphocytes of PBMC culture has the potential to be as biomarkers for the detection and therapies of autoimmune diseases.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Gene Expression
Gene Knockdown Techniques
Humans
Intracellular Signaling Peptides and Proteins genetics
Leukocytes, Mononuclear immunology
Leukocytes, Mononuclear metabolism
Lipogenesis drug effects
Lipogenesis genetics
Mechanistic Target of Rapamycin Complex 2
Models, Biological
Multiprotein Complexes metabolism
Proto-Oncogene Proteins c-akt antagonists & inhibitors
Proto-Oncogene Proteins c-akt metabolism
Homeostasis
Inflammation immunology
Inflammation metabolism
Intracellular Signaling Peptides and Proteins metabolism
Lipid Metabolism
Lymphocytes immunology
Lymphocytes metabolism
Signal Transduction
TOR Serine-Threonine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2314-7156
- Volume :
- 2017
- Database :
- MEDLINE
- Journal :
- Journal of immunology research
- Publication Type :
- Academic Journal
- Accession number :
- 28349073
- Full Text :
- https://doi.org/10.1155/2017/5252840