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Initial investigation of glucose metabolism in mouse brain using enriched 17 O-glucose and dynamic 17 O-MRS.
- Source :
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NMR in biomedicine [NMR Biomed] 2017 Aug; Vol. 30 (8). Date of Electronic Publication: 2017 Apr 03. - Publication Year :
- 2017
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Abstract
- In this initial work, the in vivo degradation of <superscript>17</superscript> O-labeled glucose was studied during cellular glycolysis. To monitor cellular glucose metabolism, direct <superscript>17</superscript> O-magnetic resonance spectroscopy (MRS) was used in the mouse brain at 9.4 T. Non-localized spectra were acquired with a custom-built transmit/receive (Tx/Rx) two-turn surface coil and a free induction decay (FID) sequence with a short TR of 5.4 ms. The dynamics of labeled oxygen in the anomeric 1-OH and 6-CH <subscript>2</subscript> OH groups was detected using a Hankel-Lanczos singular value decomposition (HLSVD) algorithm for water suppression. Time-resolved <superscript>17</superscript> O-MRS (temporal resolution, 42/10.5 s) was performed in 10 anesthetized (1.25% isoflurane) mice after injection of a 2.2 M solution containing 2.5 mg/g body weight of differently labeled <superscript>17</superscript> O-glucose dissolved in 0.9% physiological saline. From a pharmacokinetic model fit of the H <subscript>2</subscript> <superscript>17</superscript> O concentration-time course, a mean apparent cerebral metabolic rate of <superscript>17</superscript> O-labeled glucose in mouse brain of CMR <subscript>Glc</subscript>  = 0.07 ± 0.02 μmol/g/min was extracted, which is of the same order of magnitude as a literature value of 0.26 ± 0.06 μmol/g/min reported by <superscript>18</superscript> F-fluorodeoxyglucose ( <superscript>18</superscript> F-FDG) positron emission tomography (PET). In addition, we studied the chemical exchange kinetics of aqueous solutions of <superscript>17</superscript> O-labeled glucose at the C1 and C6 positions with dynamic <superscript>17</superscript> O-MRS. In conclusion, the results of the exchange and in vivo experiments demonstrate that the C6- <superscript>17</superscript> OH label in the 6-CH <subscript>2</subscript> OH group is transformed only glycolytically by the enzyme enolase into the metabolic end-product H <subscript>2</subscript> <superscript>17</superscript> O, whereas C1- <superscript>17</superscript> OH ends up in water via direct hydrolysis as well as glycolysis. Therefore, dynamic <superscript>17</superscript> O-MRS of highly labeled <superscript>17</superscript> O-glucose could provide a valuable non-radioactive alternative to FDG PET in order to investigate glucose metabolism.<br /> (Copyright © 2017 John Wiley & Sons, Ltd.)
Details
- Language :
- English
- ISSN :
- 1099-1492
- Volume :
- 30
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- NMR in biomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 28370576
- Full Text :
- https://doi.org/10.1002/nbm.3724