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Contractile properties of chemically skinned fibers from pregnant rat myometrium: existence of an internal Ca-store.
- Source :
-
Pflugers Archiv : European journal of physiology [Pflugers Arch] 1988 Mar; Vol. 411 (3), pp. 296-303. - Publication Year :
- 1988
-
Abstract
- Smooth muscle fibers isolated from pregnant rat myometrium were skinned by saponin treatment. Properties of the contractile system and the involvement of an intracellular source of activator calcium were studied. (1) In the presence of 4 mM total EGTA, skinned fibers contracted in a concentration-dependent manner to micromolar applications of calcium ions. Rat myometrium exhibited a high calcium sensitivity (80% of the maximum contraction was achieved in the presence of 10(-6) M Ca2+). (2) Several divalent cations induced concentration-dependent contractile responses in skinned uterine muscle. The rank order of potency was: Ca2+ greater than Mn2+ greater than Sr2+ greater than Ba2+. All these divalent cation-activated contractions were antagonized by trifluoperazine in a concentration-dependent manner. (3) Pretreatment of skinned fibers with cAMP (5 X 10(-5)-5 X 10(-4) M) depressed cation-activated contractions. This effect was dependent on the free cation concentration. (4) In the presence of a low EGTA concentration (0.1 mM) the pCa-tension curve was shifted to the right with a 2.5-fold increase in the Ca-concentration required to induce half-maximum contraction. (5) After Ca-loading (10(-6) M Ca2+ for 3 min in low EGTA-containing solution), total replacement of K+ ions by choline induced a small and tonic contraction. In these conditions, the ionophore A23287 (5 X 10(-8) M-5 X 10(-5) M) and inositol 1,4,5-triphosphate (InsP3; 2 X 10(-6) M-2 X 10(-5) M) also produced contractions of skinned uterine fibers even in the presence of NaN3 (5 mM) and of NaCN (5 mM).(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Animals
Calcimycin pharmacology
Calcium pharmacology
Cations, Divalent pharmacology
Choline pharmacology
Cyclic AMP pharmacology
Female
Inositol 1,4,5-Trisphosphate
Inositol Phosphates pharmacology
Potassium metabolism
Potassium pharmacology
Pregnancy
Rats
Trifluoperazine pharmacology
Calcium physiology
Myometrium drug effects
Uterine Contraction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0031-6768
- Volume :
- 411
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pflugers Archiv : European journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 2837724
- Full Text :
- https://doi.org/10.1007/BF00585118