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Antioxidant Agent Quercetin Prevents Impairment of Bladder Tissue Contractility and Apoptosis in a Rat Model of Ischemia/Reperfusion Injury.
- Source :
-
Lower urinary tract symptoms [Low Urin Tract Symptoms] 2017 May; Vol. 9 (2), pp. 117-123. Date of Electronic Publication: 2015 Dec 01. - Publication Year :
- 2017
-
Abstract
- Objectives: To examine the possible protective effect of quercetin (QT), which is well known for its antioxidant and protective effects in circumstances of oxidative stress, on urinary bladder tissue in a rat model of ischemia/reperfusion (I/R) injury, which is a known factor for the development of lower urinary tract dysfunction partly mediated by the generation of free radicals causing oxidative damage.<br />Methods: Thirty male Sprague-Dawley rats were subjected to I/R injury through clamping the abdominal aorta for 30 min and then allowing reperfusion for the next 60 min. Quercetin (20 mg/kg; subcutaneously) or vehicle were given before ischemia and just before reperfusion. Findings of the isometric contraction studies in the organ bath and of the histological examinations along with oxidative stress markers were evaluated in bladder tissues.<br />Results: Increased malondialdehyde (MDA) levels and myeloperoxidase (MPO) activities and decreased glutathione (GSH) levels and superoxide dismutase (SOD) activities in the I/R group were reduced by QT treatment. In the I/R group, pro-apoptotic marker caspase-3 was increased and anti-apoptotic bcl-2 protein was decreased, while QT treatment significantly reversed these parameters. In the I/R group contractile responses of the bladder strips to carbachol were significantly lower than those of the control group, which were reversed by QT treatment.<br />Conclusion: Quercetin treatment protects bladder tissue contractility against acute I/R injury by decreasing oxidative stress and apoptosis induced by I/R.<br /> (© 2015 Wiley Publishing Asia Pty Ltd.)
- Subjects :
- Animals
Caspases metabolism
Glutathione metabolism
Isometric Contraction drug effects
Male
Malondialdehyde metabolism
Muscle Contraction drug effects
Peroxidase metabolism
Proto-Oncogene Proteins c-bcl-2 metabolism
Rats, Sprague-Dawley
Superoxide Dismutase metabolism
Antioxidants pharmacology
Apoptosis drug effects
Quercetin pharmacology
Reperfusion Injury prevention & control
Urinary Bladder drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1757-5672
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Lower urinary tract symptoms
- Publication Type :
- Academic Journal
- Accession number :
- 28394499
- Full Text :
- https://doi.org/10.1111/luts.12125