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Progesterone Decreases in vitro Indoleamine 2, 3-dioxygenase Expression in Dendritic and CD4 + Cells from Maternal-Fetal Interface of Rats.
- Source :
-
Immunological investigations [Immunol Invest] 2017 Jul; Vol. 46 (5), pp. 447-459. Date of Electronic Publication: 2017 Apr 17. - Publication Year :
- 2017
-
Abstract
- Problem: Several mechanisms contribute to the tolerogenic state observed during pregnancy, such as the activity of the enzyme indoleamine 2, 3-dioxygenase (IDO). This initializes the catabolism of tryptophan, inducing T cells to apoptosis due to its deprivation and by the action of its catabolites in the placental microenvironment. Progesterone plays an important part on immunological tolerance mechanisms during pregnancy; however, there is no evidence it is related to IDO activity. Thus, this study aimed to investigate progesterone influence on the maternal-fetal interface of pregnant Wistar rats, by identifying IDO positive cells by immunophenotyping and flow cytometry under exogenous progesterone supplementation.<br />Method of Study: Placenta and embryo cells were cultured and separated into groups that received interferon γ or progesterone, supplemented or not with mifepristone. After 2 and 24 h, these were labeled with an anti-IDO and a series of antibodies specific to leucocytes and progesterone receptor and processed through flow cytometry analysis.<br />Results: Progesterone induced a significant decrease in the expression of IDO in dendritic cells and CD4 <superscript>+</superscript> lymphocytes.<br />Conclusion: The blocking of progesterone receptor on these cells by mifepristone restored IDO expression levels and may constitute evidence of the participation of this hormone through a direct route in these cells.
- Subjects :
- Abortifacient Agents, Steroidal pharmacology
Animals
Apoptosis drug effects
Apoptosis genetics
CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes immunology
Dendritic Cells cytology
Dendritic Cells drug effects
Dendritic Cells immunology
Embryo, Mammalian
Female
Gene Expression Regulation
Immunophenotyping
Indoleamine-Pyrrole 2,3,-Dioxygenase antagonists & inhibitors
Indoleamine-Pyrrole 2,3,-Dioxygenase genetics
Interferon-gamma pharmacology
Maternal-Fetal Exchange immunology
Mifepristone pharmacology
Placenta cytology
Placenta immunology
Pregnancy
Primary Cell Culture
Rats
Rats, Wistar
Receptors, Progesterone antagonists & inhibitors
Receptors, Progesterone genetics
Receptors, Progesterone immunology
Tryptophan immunology
Tryptophan metabolism
Uterus cytology
Uterus immunology
CD4-Positive T-Lymphocytes drug effects
Immune Tolerance drug effects
Indoleamine-Pyrrole 2,3,-Dioxygenase immunology
Placenta drug effects
Progesterone pharmacology
Uterus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1532-4311
- Volume :
- 46
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Immunological investigations
- Publication Type :
- Academic Journal
- Accession number :
- 28414589
- Full Text :
- https://doi.org/10.1080/08820139.2017.1296856