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Upregulation of CD72 expression on CD19 + CD27 + memory B cells by CD40L in primary immune thrombocytopenia.
- Source :
-
British journal of haematology [Br J Haematol] 2017 Jul; Vol. 178 (2), pp. 308-318. Date of Electronic Publication: 2017 Apr 17. - Publication Year :
- 2017
-
Abstract
- CD72 is a co-receptor of B cells and regulates B cell activation. Although aberrant expression of CD72 has been reported in primary immune thrombocytopenia (ITP), it is uncertain whether this aberrant expression is restricted to specific B cell subsets. Furthermore, the mechanisms that regulate CD72 expression are unknown. In this study, we found higher frequency of CD19 <superscript>+</superscript> B cells, CD19 <superscript>+</superscript> CD27 <superscript>+</superscript> memory B cells and lower frequency of CD19 <superscript>+</superscript> CD27 <superscript>-</superscript> naive B cells in active ITP patients compared with controls and patients in remission. CD72 expression on CD19 <superscript>+</superscript> CD27 <superscript>+</superscript> cells was upregulated in active ITP patients and correlated with platelet count and anti-platelet autoantibodies. In vitro, CD40L could specifically induce CD72 upregulation on CD19 <superscript>+</superscript> CD27 <superscript>+</superscript> B cells. In combination with CD40L, interleukin (IL) 10 and BAFF (also termed TNFSF13B) further enhanced CD72 expression on CD19 <superscript>+</superscript> CD27 <superscript>+</superscript> B cells, whereas IL21 reduced CD72 upregulation. CD72mRNA expression after CD40L stimulation was increased in ITP patients and controls. Significant increase of CD40L on CD4 <superscript>+</superscript> T cells was correlated with CD72 expression on CD19 <superscript>+</superscript> CD27 <superscript>+</superscript> B cells in ITP patients. In conclusion, upregulation of CD72 expression on CD27 <superscript>+</superscript> memory B cells might take part in the pathogenesis of ITP. Elevated CD40L on CD4 <superscript>+</superscript> cells combined with cytokines might contribute to the upregulation of CD72 expression on CD27 <superscript>+</superscript> memory B cells.<br /> (© 2017 John Wiley & Sons Ltd.)
- Subjects :
- Adult
B-Cell Activating Factor pharmacology
B-Lymphocyte Subsets immunology
CD40 Ligand pharmacology
Case-Control Studies
Cells, Cultured
Female
Humans
Immunologic Memory physiology
Interleukin-10 pharmacology
Interleukins pharmacology
Male
Middle Aged
Up-Regulation immunology
Young Adult
Antigens, CD metabolism
Antigens, CD19 metabolism
Antigens, Differentiation, B-Lymphocyte metabolism
B-Lymphocytes immunology
CD40 Ligand metabolism
Purpura, Thrombocytopenic, Idiopathic immunology
Tumor Necrosis Factor Receptor Superfamily, Member 7 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2141
- Volume :
- 178
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- British journal of haematology
- Publication Type :
- Academic Journal
- Accession number :
- 28419421
- Full Text :
- https://doi.org/10.1111/bjh.14671