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Immune response after autologous hematopoietic stem cell transplantation in type 1 diabetes mellitus.
- Source :
-
Stem cell research & therapy [Stem Cell Res Ther] 2017 Apr 18; Vol. 8 (1), pp. 90. Date of Electronic Publication: 2017 Apr 18. - Publication Year :
- 2017
-
Abstract
- Background: This study explored the details of the immune response after autologous hematopoietic stem cell transplantation (AHSCT) treatment in type 1 diabetes mellitus.<br />Methods: Peripheral blood mononuclear cells (PBMCs) from 18 patients with type 1 diabetes mellitus were taken at baseline and 12 months after AHSCT or insulin-only therapy. The lymphocyte proliferation, mRNA expression and secretion of pro-inflammatory and anti-inflammatory cytokines belonging to T-helper type 1 (Th1), T-helper type 17 (Th17) and regulatory T (Treg) cells in PBMC culture supernatants were assessed.<br />Results: Compared with patients receiving insulin-only treatment, the patients receiving AHSCT treatment showed better residual C-peptide secretion, lower anti-GAD titers and less exogenous insulin dosages after 12 months of follow-up. AHSCT treatment was associated with significantly reduced Th1 and Th17 cell proportions as well as decreased IFN-γ, IL-2, IL-12p40 and IL-17A levels in the PBMC culture supernatants (all P < 0.05). Although there was no significant Treg cell expansion after AHSCT treatment, we observed increased IL-10, TGF-β and Foxp3 mRNA expression and increased TGF-β levels. However, we found no significant changes in the T-cell subpopulations after insulin treatment, except for higher IL-12p40 mRNA expression and a lower proportion of Treg cells.<br />Conclusions: AHSCT treatment was associated with decreased expansion and function of Th1 and Th17 cells, which may explain the better therapeutic effect of AHSCT compared with the traditional intensive insulin therapy.<br />Trial Registration: Clinicaltrials.gov NCT00807651 . Registered 18 December 2008.
- Subjects :
- Adolescent
Cells, Cultured
Cytokines genetics
Cytokines metabolism
Female
Forkhead Transcription Factors genetics
Forkhead Transcription Factors metabolism
Humans
Male
Th1 Cells cytology
Th17 Cells cytology
Transplantation, Autologous adverse effects
Young Adult
Diabetes Mellitus, Type 1 therapy
Hematopoietic Stem Cell Transplantation adverse effects
Th1 Cells immunology
Th17 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1757-6512
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Stem cell research & therapy
- Publication Type :
- Academic Journal
- Accession number :
- 28420440
- Full Text :
- https://doi.org/10.1186/s13287-017-0542-1