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COPII-coated membranes function as transport carriers of intracellular procollagen I.

Authors :
Gorur A
Yuan L
Kenny SJ
Baba S
Xu K
Schekman R
Source :
The Journal of cell biology [J Cell Biol] 2017 Jun 05; Vol. 216 (6), pp. 1745-1759. Date of Electronic Publication: 2017 Apr 20.
Publication Year :
2017

Abstract

The coat protein complex II (COPII) is essential for the transport of large cargo, such as 300-nm procollagen I (PC1) molecules, from the endoplasmic reticulum (ER) to the Golgi. Previous work has shown that the CUL3-KLHL12 complex increases the size of COPII vesicles at ER exit sites to more than 300 nm in diameter and accelerates the secretion of PC1. However, the role of large COPII vesicles as PC1 transport carriers was not unambiguously demonstrated. In this study, using stochastic optical reconstruction microscopy, correlated light electron microscopy, and live-cell imaging, we demonstrate the existence of mobile COPII-coated vesicles that completely encapsulate the cargo PC1 and are physically separated from ER. We also developed a cell-free COPII vesicle budding reaction that reconstitutes the capture of PC1 into large COPII vesicles. This process requires COPII proteins and the GTPase activity of the COPII subunit SAR1. We conclude that large COPII vesicles are bona fide carriers of PC1.<br /> (© 2017 Gorur et al.)

Details

Language :
English
ISSN :
1540-8140
Volume :
216
Issue :
6
Database :
MEDLINE
Journal :
The Journal of cell biology
Publication Type :
Academic Journal
Accession number :
28428367
Full Text :
https://doi.org/10.1083/jcb.201702135