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Synergy Between Choroid Plexus Epithelial Cell-Conditioned Medium and Knockout Serum Replacement Converts Human Adipose-Derived Stem Cells to Dopamine-Secreting Neurons.
- Source :
-
Rejuvenation research [Rejuvenation Res] 2017 Aug; Vol. 20 (4), pp. 309-319. Date of Electronic Publication: 2017 Apr 27. - Publication Year :
- 2017
-
Abstract
- Human adipose-derived stem cells (hADSCs) have great capacity to differentiate into mesodermal origins as well as nonmesodermal lineages, including neural cells. This valuable feature paves the way for the therapeutic application of hADSCs for neurodegenerative maladies such as Parkinson's disease (PD). We tested the capacity of choroid plexus epithelial cell-conditioned medium (CPEC-CM) alone or cocktailed with knockout serum (KS) to induce dopaminergic (DAergic) differentiation of hADSCs. To this end, hADSCs from lipoaspirate were phenotypically characterized and shown to maintain mesodermal multipotency so that selected media easily differentiated them into osteoblasts, chondrocytes, and adipocytes. To begin inducing hADSC neuronal differentiation, we isolated CPECs from rat brain and expanded them in culture to obtain CPEC-CM. We then treated hADSCs with optimized quantities of collected CPEC-CM, KS, or both. The ADSCs treated with either CPEC-CM or CPEC-CM and KS displayed morphological changes typical of neuron-like phenotypes. As revealed by reverse transcription polymerase chain reaction (RT-PCR), quantitative real-time PCR (qPCR), and immunostaining analyses, hADSCs cotreated with CPEC-CM and KS expressed significantly higher levels of neuronal and DAergic markers in comparison with single-treated groups. Moreover, the hADSCs began expressing dopamine-biosynthesizing enzymes mainly after cotreatment with CPEC-CM and KS. Consequently, only cotreated hADSCs were capable of synthesizing and releasing dopamine detectable by high-performance liquid chromatography (HPLC). Finally, hADSCs growing in an ordinary medium were found positive for astrocytic marker glial fibrillary acidic protein (GFAP), but stopped GFAP expression on either single or cotreatments. These combined results suggest that CPEC-CM and KS can synergize to remarkably augment DAergic induction of hADSCs, an effect that has implications for cell replacement therapy for PD and related disorders.
- Subjects :
- Adipogenesis drug effects
Adult
Animals
Biomarkers metabolism
Cell Lineage drug effects
Cell Separation
Cell Shape drug effects
Chondrogenesis drug effects
Chromatography, High Pressure Liquid
Epithelial Cells drug effects
Epithelial Cells metabolism
Female
Humans
Middle Aged
Neurons drug effects
Osteogenesis drug effects
Rats
Stem Cells drug effects
Stem Cells metabolism
Young Adult
Adipose Tissue cytology
Choroid Plexus cytology
Culture Media, Conditioned pharmacology
Dopamine metabolism
Epithelial Cells cytology
Neurons metabolism
Serum metabolism
Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8577
- Volume :
- 20
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Rejuvenation research
- Publication Type :
- Academic Journal
- Accession number :
- 28437187
- Full Text :
- https://doi.org/10.1089/rej.2016.1887