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[Effects of Quercetin on Chronic Myeloid Leukemia Cell Line Resistant to Imatinib and Its Mechanism].
- Source :
-
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2017 Apr; Vol. 25 (2), pp. 346-352. - Publication Year :
- 2017
-
Abstract
- Objective: To explore the growth inhibitory effect of quercetin on imatinib-resistant chronic myeloid leukemia cell lines and to clarify its involved mechanisms.<br />Methods: The cell viability was detected by trypan blue Staining, percentage of apoptotic cells and cell cycle distribution were detected by flow cytometry, the protein expression was detected by Western blot.<br />Results: Both inhibitory effect of proliferation and apoptosis-inducing effect were similar between the imatinib-resistant and -sensitive cell lines treated with 25 µmol/L quercetin for 24 hours and with arrest of cell cycle at G <subscript>2</subscript> /M phase. Quercetin could not change the expression of BCR-ABL. The expression of γ-H2AX was markedly enhanced and the phosphorylation of JNK up-regulated by quercetin in both imatinib-resistant and imatinib-sensitive cell lines.<br />Conclusion: The growth of imatinib-resistant cells can be inhibited by quercetin, and the apoptosis of cells can be induced by quercetin, which may be related to cell cycle arrest in G <subscript>2</subscript> /M. The DNA damage and up-regulation of p-JNK may be involved in these processes.
- Subjects :
- Benzamides
Cell Line
DNA Damage drug effects
Fusion Proteins, bcr-abl
Humans
MAP Kinase Kinase 4 drug effects
MAP Kinase Kinase 4 metabolism
Piperazines
Pyrimidines
Antioxidants pharmacology
Apoptosis drug effects
Drug Resistance, Neoplasm
Imatinib Mesylate pharmacology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive drug therapy
Quercetin pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1009-2137
- Volume :
- 25
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Zhongguo shi yan xue ye xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 28446273
- Full Text :
- https://doi.org/10.7534/j.issn.1009-2137.2017.02.007