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Transcriptome-wide N 6 -methyladenosine methylome profiling of porcine muscle and adipose tissues reveals a potential mechanism for transcriptional regulation and differential methylation pattern.
- Source :
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BMC genomics [BMC Genomics] 2017 Apr 28; Vol. 18 (1), pp. 336. Date of Electronic Publication: 2017 Apr 28. - Publication Year :
- 2017
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Abstract
- Background: N <superscript>6</superscript> -methyladenosine (m <superscript>6</superscript> A) is the most prevalent internal form of modification in messenger RNA in higher eukaryotes and potential regulatory functions of reversible m <superscript>6</superscript> A methylation on mRNA have been revealed by mapping of m <superscript>6</superscript> A methylomes in several species. m <superscript>6</superscript> A modification in active gene regulation manifests itself as altered methylation profiles in a tissue-specific manner or in response to changing cellular or species living environment. However, up to date, there has no data on m <superscript>6</superscript> A porcine transcriptome-wide map and its potential biological roles in adipose deposition and muscle growth.<br />Methods: In this work, we used methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-Seq) technique to acquire the first ever m <superscript>6</superscript> A porcine transcriptome-wide map. Transcriptomes of muscle and adipose tissues from three different pig breeds, the wild boar, Landrace, and Rongchang pig, were used to generate these maps.<br />Results: Our findings show that there were 5,872 and 2,826 m <superscript>6</superscript> A peaks respectively, in the porcine muscle and adipose tissue transcriptomes. Stop codons, 3'-untranslated regions, and coding regions were found to be mainly enriched for m <superscript>6</superscript> A peaks. Gene ontology analysis revealed that common m <superscript>6</superscript> A peaks in nuclear genes are associated with transcriptional factors, suggestive of a relationship between m <superscript>6</superscript> A mRNA methylation and nuclear genome transcription. Some genes showed tissue- and breed-differential methylation, and have novel biological functions. We also found a relationship between the m <superscript>6</superscript> A methylation extent and the transcript level, suggesting a regulatory role for m <superscript>6</superscript> A in gene expression.<br />Conclusion: This comprehensive map provides a solid basis for the determination of potential functional roles for RNA m <superscript>6</superscript> A modification in adipose deposition and muscle growth.
Details
- Language :
- English
- ISSN :
- 1471-2164
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC genomics
- Publication Type :
- Academic Journal
- Accession number :
- 28454518
- Full Text :
- https://doi.org/10.1186/s12864-017-3719-1