Back to Search
Start Over
A heterologous prime-boosting strategy with replicating Vaccinia virus vectors and plant-produced HIV-1 Gag/dgp41 virus-like particles.
- Source :
-
Virology [Virology] 2017 Jul; Vol. 507, pp. 242-256. Date of Electronic Publication: 2017 Apr 28. - Publication Year :
- 2017
-
Abstract
- Showing modest efficacy, the RV144 HIV-1 vaccine clinical trial utilized a non-replicating canarypox viral vector and a soluble gp120 protein boost. Here we built upon the RV144 strategy by developing a novel combination of a replicating, but highly-attenuated Vaccinia virus vector, NYVAC-KC, and plant-produced HIV-1 virus-like particles (VLPs). Both components contained the full-length Gag and a membrane anchored truncated gp41 presenting the membrane proximal external region with its conserved broadly neutralizing epitopes in the pre-fusion conformation. We tested different prime/boost combinations of these components in mice and showed that the group primed with NYVAC-KC and boosted with both the viral vectors and plant-produced VLPs have the most robust Gag-specific CD8 T cell responses, at 12.7% of CD8 T cells expressing IFN-γ in response to stimulation with five Gag epitopes. The same immunization group elicited the best systemic and mucosal antibody responses to Gag and dgp41 with a bias towards IgG1.<br /> (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- AIDS Vaccines immunology
Animals
Antibodies, Neutralizing immunology
Antibody Formation
Female
Genetic Vectors genetics
Genetic Vectors physiology
HIV Antibodies immunology
HIV Envelope Protein gp41 administration & dosage
HIV Envelope Protein gp41 genetics
HIV Infections prevention & control
HIV Infections virology
HIV-1 genetics
Humans
Immunization, Secondary
Mice
Mice, Inbred C57BL
Nicotiana genetics
Nicotiana virology
Vaccines, Virus-Like Particle genetics
Vaccines, Virus-Like Particle immunology
Vaccinia virus genetics
Virus Replication
gag Gene Products, Human Immunodeficiency Virus administration & dosage
gag Gene Products, Human Immunodeficiency Virus genetics
AIDS Vaccines administration & dosage
HIV Envelope Protein gp41 immunology
HIV Infections immunology
HIV-1 immunology
Immunization methods
Nicotiana metabolism
Vaccinia virus physiology
gag Gene Products, Human Immunodeficiency Virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 507
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 28458036
- Full Text :
- https://doi.org/10.1016/j.virol.2017.04.008