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The effects of the stem cell on ciliary regeneration of injured rabbit sinonasal epithelium.
- Source :
-
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery [Eur Arch Otorhinolaryngol] 2017 Aug; Vol. 274 (8), pp. 3057-3064. Date of Electronic Publication: 2017 May 02. - Publication Year :
- 2017
-
Abstract
- Defects in mucosal healing after sinonasal surgery cause infection, scar formation causing obstruction, relapse of the disease within a shorter period and revision surgery. The present study aimed to create a functional ciliated epithelium using a stem cell and stem cell sheet of adipose tissue origin and to show such regeneration ultra-structurally on experimentally injured rabbit nasal epithelium. This was an experimental animal study and basic research. A total of 18 white New Zealand rabbits were divided into three groups. The medial wall of the maxillary sinus of the subjects was peeled off bilaterally. No additional procedure was applied to the subjects in Group 1. In Group 2, adipose tissue-derived mesenchymal stem cell was implanted on the wound edges of the subjects. In Group 3, a stem cell sheet of three layers was laid onto the defect area. All subjects were killed after 3 weeks. The presence of the stem cell stained with bromo-deoxyuridine was assessed with a light microscope, whereas cilia density, ciliated orientation and cilia structure were evaluated with a scanning electron microscope. Ciliary densities in Group 2 and Group 3 were statistically superior compared to the control group (p < 0.001, p = 0.007). Cilia morphology in Group 2 and Group 3 was also better than the control group (p < 0.01, p = 0.048). Ciliary orientation in Group 2 was scored highest (p < 0.01). The ratio of BrDu-stained cells was observed to be 27% in Group 3 and 8% in Group 2. Sub-epithelial recovery was observed to be better in Group 3. Adipose tissue-derived mesenchymal stem cell increased the healing of the injured maxillary sinus mucosa of the rabbits in terms of cilia presence, density and morphology regardless of the implementation technique. Level of evidence NA.
- Subjects :
- Adipose Tissue cytology
Animals
Male
Maxillary Sinus pathology
Maxillary Sinus physiopathology
Maxillary Sinus surgery
Models, Animal
Nasal Surgical Procedures
Rabbits
Treatment Outcome
Cilia physiology
Mesenchymal Stem Cell Transplantation methods
Mesenchymal Stem Cells physiology
Nasal Mucosa injuries
Nasal Mucosa pathology
Nasal Mucosa physiopathology
Wound Healing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1434-4726
- Volume :
- 274
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 28466357
- Full Text :
- https://doi.org/10.1007/s00405-017-4595-7