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Biomechanical and Histopathologic Effects of Pulsed-Light Accelerated Epithelium-On/-Off Corneal Collagen Cross-Linking.
- Source :
-
Cornea [Cornea] 2017 Jul; Vol. 36 (7), pp. 854-859. - Publication Year :
- 2017
-
Abstract
- Purpose: This study aimed to compare the biomechanical and histopathologic effects of transepithelial and accelerated epithelium-off pulsed-light accelerated corneal collagen cross-linking (CXL).<br />Methods: A total of 24 New Zealand rabbits were analyzed after sham operation (control) or transepithelial or epithelium-off operation (45 mW/cm for both). The transepithelial group was treated with pulsed-light ultraviolet A for 5 minutes 20 seconds, and the epithelium-off group was treated for 90 seconds. Biomechanical testing, including ultimate stress, Young modulus, and the physiological modulus, was analyzed. Histological changes were evaluated by light microscopy and transmission electron microscopy.<br />Results: The stress-strain curve was nonlinear in both accelerated transepithelial and epithelium-off CXL groups. The stress and elastic moduli were all significantly higher in both experimental groups compared with the control group (P < 0.05), whereas there were no significant differences between the 2 treatment groups (P > 0.05). Six months after the operation, hematoxylin and eosin staining and transmission electron microscopy showed that the subcutaneous collagen fibers were arranged in a regular pattern, and the fiber density was higher in the experimental groups.<br />Conclusions: Both transepithelial and accelerated epithelium-off CXL produced biomechanical and histopathologic improvements, which were not significantly different between the 2 pulsed-light accelerated CXL treatments.
- Subjects :
- Animals
Collagen ultrastructure
Cornea physiology
Corneal Stroma ultrastructure
Debridement
Elastic Modulus
Elasticity physiology
Rabbits
Ultraviolet Rays
Collagen metabolism
Corneal Stroma metabolism
Cross-Linking Reagents
Epithelium, Corneal metabolism
Photochemotherapy methods
Photosensitizing Agents therapeutic use
Riboflavin therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4798
- Volume :
- 36
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cornea
- Publication Type :
- Academic Journal
- Accession number :
- 28486312
- Full Text :
- https://doi.org/10.1097/ICO.0000000000001219