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Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.
- Source :
-
Genes to cells : devoted to molecular & cellular mechanisms [Genes Cells] 2017 Jun; Vol. 22 (6), pp. 552-567. Date of Electronic Publication: 2017 May 12. - Publication Year :
- 2017
-
Abstract
- In meiosis I, sister chromatids are captured by microtubules emanating from the same pole (mono-orientation), and centromeric cohesion is protected throughout anaphase. Shugoshin, which is localized to centromeres depending on the phosphorylation of histone H2A by Bub1 kinase, plays a central role in protecting meiotic cohesin Rec8 from separase cleavage. Another key meiotic kinetochore factor, meikin, may regulate cohesion protection, although the underlying molecular mechanisms remain elusive. Here, we show that fission yeast Moa1 (meikin), which associates stably with CENP-C during meiosis I, recruits Plo1 (polo-like kinase) to the kinetochores and phosphorylates Spc7 (KNL1) to accumulate Bub1. Consequently, in contrast to the transient kinetochore localization of mitotic Bub1, meiotic Bub1 persists at kinetochores until anaphase I. The meiotic Bub1 pool ensures robust Sgo1 (shugoshin) localization and cohesion protection at centromeres by cooperating with heterochromatin protein Swi6, which binds and stabilizes Sgo1. Furthermore, molecular genetic analyses show a hierarchical regulation of centromeric cohesion protection by meikin and shugoshin that is important for establishing meiosis-specific chromosome segregation. We provide evidence that the meiosis-specific Bub1 regulation is conserved in mouse.<br /> (© 2017 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.)
- Subjects :
- Animals
Cell Adhesion
Cells, Cultured
Centromere genetics
Centromere metabolism
Chromosomal Proteins, Non-Histone metabolism
Chromosomal Proteins, Non-Histone physiology
Kinetochores
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Microtubules metabolism
Phosphorylation
Schizosaccharomyces cytology
Schizosaccharomyces growth & development
Spermatocytes cytology
Spermatocytes metabolism
Polo-Like Kinase 1
Cell Cycle Proteins metabolism
Gene Expression Regulation, Fungal
Meiosis
Protein Serine-Threonine Kinases metabolism
Proto-Oncogene Proteins metabolism
Schizosaccharomyces metabolism
Schizosaccharomyces pombe Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2443
- Volume :
- 22
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Genes to cells : devoted to molecular & cellular mechanisms
- Publication Type :
- Academic Journal
- Accession number :
- 28497540
- Full Text :
- https://doi.org/10.1111/gtc.12496