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Phosphorylation of the transcriptional repressor MYB15 by mitogen-activated protein kinase 6 is required for freezing tolerance in Arabidopsis.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2017 Jun 20; Vol. 45 (11), pp. 6613-6627. - Publication Year :
- 2017
-
Abstract
- The expression of CBF (C-repeat-binding factor) genes is required for freezing tolerance in Arabidopsis thaliana. CBFs are positively regulated by INDUCER OF CBF EXPRESSION1 (ICE1) and negatively regulated by MYB15. These transcription factors directly interact with specific elements in the CBF promoters. Mitogen-activated protein kinase (MAPK/MPK) cascades function upstream to regulate CBFs. However, the mechanism by which MPKs control CBF expression during cold stress signaling remains unknown. This study showed that the activity of MYB15, a transcriptional repressor of cold signaling, is regulated by MPK6-mediated phosphorylation. MYB15 specifically interacts with MPK6, and MPK6 phosphorylates MYB15 on Ser168. MPK6-induced phosphorylation reduced the affinity of MYB15 binding to the CBF3 promoter and mutation of its phosphorylation site (MYB15S168A) enhanced the transcriptional repression of CBF3 by MYB15. Furthermore, transgenic plants overexpressing MYB15S168A showed significantly reduced CBF transcript levels in response to cold stress, compared with plants overexpressing MYB15. The MYB15S168A-overexpressing plants were also more sensitive to freezing than MYB15-overexpressing plants. These results suggest that MPK6-mediated regulation of MYB15 plays an important role in cold stress signaling in Arabidopsis.<br /> (© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- Adaptation, Physiological
Cold-Shock Response
DNA, Plant chemistry
DNA, Plant genetics
Enzyme Activation
Freezing
Gene Expression Regulation, Plant
MAP Kinase Signaling System
Phosphorylation
Promoter Regions, Genetic
Protein Binding
Protein Interaction Maps
Nicotiana
Up-Regulation
Arabidopsis physiology
Arabidopsis Proteins metabolism
Arabidopsis Proteins physiology
Mitogen-Activated Protein Kinases physiology
Protein Processing, Post-Translational
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 45
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 28510716
- Full Text :
- https://doi.org/10.1093/nar/gkx417