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Molecular mechanisms underlying the protective effects of hydrogen-saturated saline on noise-induced hearing loss.
- Source :
-
Acta oto-laryngologica [Acta Otolaryngol] 2017 Oct; Vol. 137 (10), pp. 1063-1068. Date of Electronic Publication: 2017 May 26. - Publication Year :
- 2017
-
Abstract
- Objectives: This study aimed to explore the molecular mechanism of the protective effects of hydrogen-saturated saline on NIHL.<br />Methods: Guinea pigs were divided into three groups: hydrogen-saturated saline; normal saline; and control. For saline administration, the guinea pigs were given daily abdominal injections 3 d before and 1 h before noise exposure. ABR were tested to examine cochlear physiology changes. The changes of 8-hydroxy-desoxyguanosine (8-HOdG), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), intercellular cell adhesion molecule-1 (ICAM-1) and high mobility group box-1 protein (HMGB1) in the cochlea were also examined.<br />Results: The results showed that pre-treatment with hydrogen-saturated saline could significantly attenuate noise-induced hearing loss. The concentration of 8-HOdG was also significantly decreased in the hydrogen-saturated saline group compared with the normal saline group. After noise exposure, the concentrations of IL-1, IL-6, TNF-α, and ICAM-1 in the cochlea of guinea pigs in the hydrogen-saturated saline group were dramatically reduced compared to those in the normal saline group. The concentrations of HMGB-1 and IL-10 in the hydrogen-saturated saline group were significantly higher than in those in the normal saline group immediately and at 7 d after noise exposure.<br />Conclusions: This study revealed for the first time the protective effects of hydrogen-saturated saline on noise-induced hearing loss (NIHL) are related to both the anti-oxidative activity and anti-inflammatory activity.
- Subjects :
- 8-Hydroxy-2'-Deoxyguanosine
Animals
Cochlea metabolism
Cytokines metabolism
Deoxyguanosine analogs & derivatives
Deoxyguanosine metabolism
Disease Models, Animal
Evoked Potentials, Auditory, Brain Stem drug effects
Female
Guinea Pigs
Hearing Loss, Noise-Induced etiology
Hearing Loss, Noise-Induced metabolism
Inflammation Mediators metabolism
Male
Sodium Chloride pharmacology
Cochlea drug effects
Hearing Loss, Noise-Induced prevention & control
Hydrogen pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1651-2251
- Volume :
- 137
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Acta oto-laryngologica
- Publication Type :
- Academic Journal
- Accession number :
- 28549396
- Full Text :
- https://doi.org/10.1080/00016489.2017.1328743