RT-PCR for Detecting ALK Translocations in Cytology Samples from Lung Cancer Patients.

Authors :: Nakamichi S
Seike M
Miyanaga A
Chiba M
Matsuda K
Kobayashi K
Takahashi A
Takeuchi S
Minegishi Y
Kubota K
Gemma A
Source :: Anticancer research [Anticancer Res] 2017 Jun; Vol. 37 (6), pp. 3295-3299.
Publication Year :: 2017
Abstract: Background/aim: We evaluated the usefulness of reverse transcription-polymerase chain reaction (RT-PCR) for detecting anaplastic lymphoma kinase (ALK) translocations using cytology samples from lung cancer patients.<br />Materials and Methods: We analyzed ALK translocations by RT-PCR in cytology samples from lung cancer patients diagnosed at the Nippon Medical School Hospital between 2013 and 2015. Immunochemistry (IHC) and break-apart fluorescence in situ hybridization (FISH) were also performed on available tissue samples.<br />Results: A total of 155 cytology samples were analyzed in our study. We obtained 115 (68%) samples from bronchial lavage. We were able to determine 153 (99%) results by RT-PCR with 4 (3%) positive samples. The four samples positive by RT-PCR were also positive by IHC and FISH performed on the tissue samples collected simultaneously.<br />Conclusion: RT-PCR is a suitable method for detecting ALK translocations using cytology samples from patients with primary lung cancer, especially when tissue samples are not available.<br /> (Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)

Full Text Access

Tools