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Combination of Helicobacter pylori infection and the interleukin 8 -251 T > A polymorphism, but not the mannose-binding lectin 2 codon 54 G > A polymorphism, might be a risk factor of gastric cancer.
- Source :
-
BMC cancer [BMC Cancer] 2017 May 30; Vol. 17 (1), pp. 388. Date of Electronic Publication: 2017 May 30. - Publication Year :
- 2017
-
Abstract
- Background: Mannose-binding lectin (MBL) acts in the innate immune response to Helicobacter pylori. Interleukin 8 (IL-8) is a potent cytokine produced by gastric epithelial cells in response to H. pylori. We aimed to investigate whether polymorphisms in MBL2 and IL-8 influence susceptibility to H. pylori infection, and the associations of these polymorphisms with the risk of gastroduodenal diseases in a Korean population.<br />Methods: We consecutively enrolled 176 H. pylori-negative control subjects, 221 subjects with H. pylori-positive non-atrophic gastritis, 52 mild atrophic gastritis (AG), 61 severe AG, 175 duodenal ulcer, and 283 gastric cancer (GC). Allele-specific PCR-RFLP was conducted for polymorphisms in MBL2 exon 1 (codon 52, 54, and 57) and IL-8 -251 T > A. IL-8 levels in gastric mucosal tissues and serum MBL levels were measured by enzyme-linked immunosorbent assay.<br />Results: MBL2 exon 1 polymorphic variants were found only in codon 54, and the allele frequencies did not differ significantly between the control and disease groups. Although serum MBL levels in codon 54 A/A mutants were markedly low, it did not influence susceptibility to H. pylori infection or the risk of gastroduodenal diseases. IL-8 levels were significantly different between T/T wild type, T/A heterozygote, and A/A mutant genotypes. IL-8 -251 A allele carriers (A/A + T/A) showed increased IL-8 levels, and were significantly associated with the risk of severe AG and GC.<br />Conclusions: We suggest that a combination of H. pylori infection and the IL-8 -251 T > A polymorphism might increase the risk of severe AG and GC in a Korean population.
- Subjects :
- Adult
Aged
Female
Gene Frequency
Genetic Association Studies
Genetic Predisposition to Disease
Genotype
Helicobacter Infections microbiology
Helicobacter Infections pathology
Helicobacter pylori pathogenicity
Humans
Male
Middle Aged
Polymorphism, Single Nucleotide genetics
Republic of Korea
Risk Factors
Stomach Neoplasms microbiology
Stomach Neoplasms pathology
Helicobacter Infections genetics
Interleukin-8 genetics
Mannose-Binding Lectin genetics
Stomach Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2407
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC cancer
- Publication Type :
- Academic Journal
- Accession number :
- 28558668
- Full Text :
- https://doi.org/10.1186/s12885-017-3378-2