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ANTITUMOR AND APOPTOTIC EFFECTS OF CUCURBITACIN A IN A-549 LUNG CARCINOMA CELLS IS MEDIATED VIA G2/M CELL CYCLE ARREST AND M-TOR/PI3K/AKT SIGNALLING PATHWAY.
- Source :
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African journal of traditional, complementary, and alternative medicines : AJTCAM [Afr J Tradit Complement Altern Med] 2017 Jan 13; Vol. 14 (2), pp. 75-82. Date of Electronic Publication: 2017 Jan 13 (Print Publication: 2017). - Publication Year :
- 2017
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Abstract
- Background: The main aim of this study was to demonstrate the antitumor potential of cucurbitacin A on A-549 NSCLC (non-small cell lung cancer cells). The effects of Cucurbitacin A on apoptotic induction, cell physic, cell cycle failure and m-TOR/PI3K/Akt signalling pathway were also investigated in the present study.<br />Materials and Methods: MTT assay and clonogenic assay were carried out to study effects of this compound on cell cytotoxicity and colony forming tendency in A-549 cells. Moreover, phase and fluorescence microscopic techniques were used to examine the effects on cell morphology and induction of apoptosis. The effects on cell cycle phase distribution were investigated by flow cytometry and effects on m-TOR/PI3K/Akt signalling proteins were assessed by western blot analysis.<br />Results: Results showed that cucurbitacin A induced dose-dependent cytotoxic effects along with suppressing the colony forming tendency in these cells. Cucurbitacin A also induced morphological changes in these cells featuring chromatin condensation, cell shrinkage and apoptotic body formation. G2/M phase cell cycle collapse was also induced by Cucurbitacin A along with inhibition of expression levels of m-TOR/PI3K/Akt proteins.<br />Conclusions: In conclusion, cucurbitacin A inhibits cancer growth in A-549 NSCLC cells by inducing apoptosis, targeting m-TOR/PI3K/Akt signalling pathway and G2/M cell cycle.<br />Competing Interests: Conflict of interest: The authors declare no conflict of interest
- Subjects :
- A549 Cells
Antineoplastic Agents, Phytogenic pharmacology
Apoptosis
Carcinoma drug therapy
Carcinoma metabolism
Cucurbitaceae chemistry
Cucurbitacins pharmacology
Humans
Lung Neoplasms metabolism
Lung Neoplasms pathology
Phosphatidylinositol 3-Kinases metabolism
Plant Extracts pharmacology
Plant Extracts therapeutic use
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction
TOR Serine-Threonine Kinases metabolism
Antineoplastic Agents, Phytogenic therapeutic use
Cell Cycle Checkpoints drug effects
Cucurbitacins therapeutic use
Lung drug effects
Lung Neoplasms drug therapy
Phosphotransferases metabolism
Phytotherapy
Subjects
Details
- Language :
- English
- ISSN :
- 2505-0044
- Volume :
- 14
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- African journal of traditional, complementary, and alternative medicines : AJTCAM
- Publication Type :
- Academic Journal
- Accession number :
- 28573224
- Full Text :
- https://doi.org/10.21010/ajtcam.v14i2.9