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Response of live Newcastle disease virus encapsulated in N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan nanoparticles.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2017 Sep 01; Vol. 171, pp. 267-280. Date of Electronic Publication: 2017 May 10. - Publication Year :
- 2017
-
Abstract
- We have synthesized N-2-hydroxypropyl dimethylethyl ammonium chloride chitosan (N-2-HFCC) with higher water solubility than chitosan. To evaluate its potential as delivery carrier, NDV encapsulated in N-2-HFCC/CMC nanoparticles (NDV/La Sota-N-2-HFCC/CMC-NPs) with a diameter of 252.2±32.68nm, Zeta potential of 41.1±0.89mV, encapsulation efficiency of 96.6±0.72% and loading capacity of 53.2±1.11% were prepared. NDV/La Sota-N-2-HFCC/CMC-NPs are a novel type of attenuated live vaccine encapsulated in nanoparticles. These nanoparticles displayed lower cytotoxicity and higher stability. Their bioactivity was maintained when they were stored for three months at the room temperature. Release assay in vitro showed that NDV could be sustainably released after an initial burst release. In vivo immunization showed that immunization of specific pathogen free chickens with NDV/La Sota-N-2-HFCC/CMC-NPs intranasally induced high titers of serum antibody, significantly promoted lymphocyte proliferation and caused higher levels of interleukine-2 and interference-γ. These results indicated that N-2-HFCC/CMC nanoparticles could serve as an efficient and safe delivery vehicle for mucosal immunity, and have a great potential in medical applications.<br /> (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Subjects :
- Ammonium Chloride chemistry
Animals
Chickens
Immunity, Mucosal immunology
Newcastle Disease prevention & control
Newcastle disease virus immunology
Specific Pathogen-Free Organisms
Vaccines, Attenuated administration & dosage
Chitosan chemistry
Immunity, Mucosal drug effects
Nanoparticles chemistry
Newcastle Disease immunology
Viral Vaccines administration & dosage
Viral Vaccines pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 171
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 28578963
- Full Text :
- https://doi.org/10.1016/j.carbpol.2017.05.022