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Acanthamoeba castellanii is not be an adequate model to study human adenovirus interactions with macrophagic cells.
- Source :
-
PloS one [PLoS One] 2017 Jun 07; Vol. 12 (6), pp. e0178629. Date of Electronic Publication: 2017 Jun 07 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- Free living amoebae (FLA) including Acanthamoeba castellanii, are protozoa that feed on different microorganisms including viruses. These microorganisms show remarkable similarities with macrophages in cellular structures, physiology or ability to phagocyte preys, and some authors have therefore wondered whether Acanthamoeba and macrophages are evolutionary related. It has been considered that this amoeba may be an in vitro model to investigate relationships between pathogens and macrophagic cells. So, we intended in this study to compare the interactions between a human adenovirus strain and A. castellanii or THP-1 macrophagic cells. The results of molecular and microscopy techniques following co-cultures experiments have shown that the presence of the adenovirus decreased the viability of macrophages, while it has no effect on amoebic viability. On another hand, the viral replication occurred only in macrophages. These results showed that this amoebal model is not relevant to explore the relationships between adenoviruses and macrophages in in vitro experiments.
- Subjects :
- Adenoviruses, Human ultrastructure
Cell Line
Cell Survival
Coculture Techniques
DNA, Viral metabolism
Fluorescent Antibody Technique
Humans
Macrophages ultrastructure
Phagocytes cytology
Trophozoites ultrastructure
Acanthamoeba castellanii physiology
Adenoviruses, Human physiology
Macrophages parasitology
Macrophages virology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 28591183
- Full Text :
- https://doi.org/10.1371/journal.pone.0178629