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miR-152 regulated glioma cell proliferation and apoptosis via Runx2 mediated by DNMT1.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2017 Aug; Vol. 92, pp. 690-695. Date of Electronic Publication: 2017 Jun 06. - Publication Year :
- 2017
-
Abstract
- Background: Aberrant DNA methylation is associated with tumor onset and progression. Study has verified that the DNA methylation of miR-152 was mediated in many tumors, but whether it involved in glioblastomas was still unclear.<br />Methods: This study enrolled 20 patients with glioma to analyze the expression pattern of miR-152. Real-time PCR and western blot were used to detect the mRNA or protein expression level, respectively. The relationship between miR-152 and runx2 was detected by Luciferase reporter assay. The methylation level of miR-152 was determined by methylation-specific PCR. Cell proliferation and apoptosis were detected by MTT and Annexin-FITC/PI assay.<br />Results: The expression of miR-152 was down-regulated while the expression of DNMT1 was up-regulated in both glioma tissue and cell lines. MiR-152 was hypermethylated and its expression was negatively correlated with DNMT in glioma cell lines. DNMT1 knockdown promoted the expression of miR-152, however, DNMT1 overexpression suppressed the expression of miR-152. MiR-152 overexpression promoted glioma cell apoptosis while miR-152 knockdown promoted cell proliferation. MiR-152 targets Runx2 to regulate its expression, Runx2 overexpression abolished the effects of miR-152 overexpression.<br />Conclusion: MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation.<br /> (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Cell Line, Tumor
Core Binding Factor Alpha 1 Subunit genetics
DNA (Cytosine-5-)-Methyltransferase 1 genetics
Glioma genetics
Humans
Apoptosis physiology
Cell Proliferation physiology
Core Binding Factor Alpha 1 Subunit biosynthesis
DNA (Cytosine-5-)-Methyltransferase 1 biosynthesis
Glioma metabolism
MicroRNAs physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 92
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 28595085
- Full Text :
- https://doi.org/10.1016/j.biopha.2017.05.096