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Molecular cloning and analysis of a C-type lectin from silkworm Bombyx mori.
- Source :
-
Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 2017 Jul; Vol. 95 (3). Date of Electronic Publication: 2017 Jun 15. - Publication Year :
- 2017
-
Abstract
- C-type lectins (CTLs) play a variety of roles in plants and animals. They are involved in animal development, pathogen recognition, and the activation of immune responses. CTLs carry one or more non-catalytic carbohydrate-recognition domains (CRDs) to bind specific carbohydrates reversibly. Here, we report the molecular cloning and functional analysis of a single-CRD CTL, named C-type lectin-S2 (BmCTL-S2) from the domesticated silkmoth Bombyx mori (Lepidoptera: Bombycidae). The ORF of CTL-S2 is 666 bp, which encodes a putative protein of 221 amino acids. BmCTL-S2 is expressed in a variety of immune-related tissues, including hemocytes and fat body among others. BmCTL-S2 mRNA level in the midgut and the fat body was significantly increased by bacterial challenges. The recombinant protein (rBmCTL-S2) bound different bacterial cell wall components and bacterial cells. rBmCTL-S2 also inhibited the growth of Bacillus subtilis and Staphylococcus aureus. Taken together, we infer that BmCTL-S2 is a pattern-recognition receptor with antibacterial activities.<br /> (© 2017 Wiley Periodicals, Inc.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Bombyx genetics
Bombyx immunology
Fat Body metabolism
Insect Proteins isolation & purification
Insect Proteins physiology
Larva immunology
Larva metabolism
Lectins, C-Type isolation & purification
Microbial Sensitivity Tests
Pathogen-Associated Molecular Pattern Molecules metabolism
Sequence Analysis, DNA
Bombyx metabolism
Lectins, C-Type physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6327
- Volume :
- 95
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Archives of insect biochemistry and physiology
- Publication Type :
- Academic Journal
- Accession number :
- 28618068
- Full Text :
- https://doi.org/10.1002/arch.21391