Back to Search
Start Over
Differentiation Affects the Release of Exosomes from Colon Cancer Cells and Their Ability to Modulate the Behavior of Recipient Cells.
- Source :
-
The American journal of pathology [Am J Pathol] 2017 Jul; Vol. 187 (7), pp. 1633-1647. Date of Electronic Publication: 2017 Jun 12. - Publication Year :
- 2017
-
Abstract
- Exosomes are involved in intercellular communication. We previously reported that sodium butyrate-induced differentiation of HT29 colon cancer cells is associated with a reduced CD133 expression. Herein, we analyzed the role of exosomes in the differentiation of HT29 cells. Exosomes were prepared using ultracentrifugation. Gene expression levels were evaluated by real-time PCR. The cell proliferation rate was assessed by MTT assay and with the electric cell-substrate impedance sensing system, whereas cell motility was assessed using the scratch test and confocal microscopy. Sodium butyrate-induced differentiation of HT29 and Caco-2 cells increased the levels of released exosomes and their expression of CD133. Cell differentiation and the decrease of cellular CD133 expression levels were prevented by blocking multivesicular body maturation. Exosomes released by HT29 differentiating cells carried increased levels of miRNAs, induced an increased proliferation and motility of both colon cancer cells and normal fibroblasts, increased the colony-forming efficiency of cancer cells, and reduced the sodium butyrate-induced differentiation of HT29 cells. Such effects were associated with an increased phosphorylation level of both Src and extracellular signal regulated kinase proteins and with an increased expression of epithelial-to-mesenchymal transition-related genes. Release of exosomes is affected by differentiation of colon cancer cells; exosomes might be used by differentiating cells to get rid of components that are no longer necessary but might continue to exert their effects on recipient cells.<br /> (Copyright © 2017 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- AC133 Antigen genetics
Butyric Acid adverse effects
Caco-2 Cells
Cell Differentiation drug effects
Cell Movement drug effects
Cell Proliferation
Colonic Neoplasms genetics
Colonic Neoplasms pathology
Exosomes genetics
Gene Expression Regulation, Neoplastic
HT29 Cells
Humans
MicroRNAs genetics
MicroRNAs metabolism
Phosphorylation
Real-Time Polymerase Chain Reaction
AC133 Antigen metabolism
Colonic Neoplasms metabolism
Exosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1525-2191
- Volume :
- 187
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 28619275
- Full Text :
- https://doi.org/10.1016/j.ajpath.2017.03.015