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Systemic Immunological changes in patients with distinct clinical outcomes during Mycobacterium tuberculosis infection.
- Source :
-
Immunobiology [Immunobiology] 2017 Nov; Vol. 222 (11), pp. 1014-1024. Date of Electronic Publication: 2017 May 26. - Publication Year :
- 2017
-
Abstract
- Background: The lung lesions in an individual infected with tuberculosis (TB) are surprisingly variable and independent of each other. However, there is no circulating biomarker yet able to segregate patients according to the extent of lung lesions.<br />Materials and Methods: In this study, the phenotypic and functional profile of leukocytes of patients with active pulmonary tuberculosis (TB) and controls (CO) were fully scrutinized by immunophenotyping assays and in vitro short-term whole blood culture. The TB group was subdivided according to the extent of lung lesions as unilateral (UNI) and bilateral (BI).<br />Results: The results show that TB group display an altered leukocyte profile in the peripheral blood with significant lower counts of NK-cells, CD3 <superscript>+</superscript> CD56 <superscript>+</superscript> CD16 <superscript>+/-</superscript> NKT-cells, CD4 <superscript>+</superscript> T-cells, CD19 <superscript>+</superscript> B-cells when compared to CO. Increased CD4 <superscript>+</superscript> T-cells and CD8 <superscript>+</superscript> T-cell activation was observed by the upregulation of activation markers (HLA-DR) as well as of chemokine receptors (CCR2, CCR3, and CXCR4). In addition, TB group presented a significant decrease proportion of CD14 <superscript>Low</superscript> CD16 <superscript>+</superscript> monocytes despite the increase in HLA-DR expression. Regarding the severity of the disease, in the BI group a reduction in frequency of CD19 <superscript>+</superscript> CD5 <superscript>+</superscript> B-cells and expression of HLA-DR in CD14 <superscript>Low</superscript> CD16 <superscript>+</superscript> monocytes was observed. Furthermore, the extent of lung lesions influences the production of molecules as observed by significantly larger production of IL-4 by neutrophils, total T-cells, CD4 <superscript>+</superscript> T-cells, CD8 <superscript>+</superscript> T-cells and CD19 <superscript>+</superscript> B-cells in UNI as compared to BI. By contrast, in BI group the frequency of high producers of both IL-17 <superscript>+</superscript> CD4 <superscript>+</superscript> T-cells and IL-17 <superscript>+</superscript> CD8 <superscript>+</superscript> T-cells were significantly increased than UNI, suggesting the deleterious role of these subsets during active pulmonary Mtb infection.<br />Conclusion: The immunophenotypic characterization of unilateral and bilateral active TB performed in the present study indicates that the extent of lung lesion could be associated with a fine-tuning between immunological responses during untreated Mtb infection.<br /> (Copyright © 2017 Elsevier GmbH. All rights reserved.)
- Subjects :
- Adult
Aged
Antigens, CD19 metabolism
CD56 Antigen metabolism
Cytokines metabolism
Female
HLA-DR Antigens metabolism
Humans
Immunity
Leukocytes, Mononuclear microbiology
Lung anatomy & histology
Lung microbiology
Male
Middle Aged
Receptors, Chemokine metabolism
Young Adult
B-Lymphocytes immunology
CD4-Positive T-Lymphocytes immunology
Killer Cells, Natural immunology
Leukocytes, Mononuclear immunology
Lung immunology
Mycobacterium tuberculosis immunology
Tuberculosis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3279
- Volume :
- 222
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 28619539
- Full Text :
- https://doi.org/10.1016/j.imbio.2017.05.016