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Comprehensive Modeling of Corneal Alkali Injury in the Rat Eye.
- Source :
-
Current eye research [Curr Eye Res] 2017 Oct; Vol. 42 (10), pp. 1348-1357. Date of Electronic Publication: 2017 Jun 21. - Publication Year :
- 2017
-
Abstract
- Purpose: To characterize the molecular, clinical, and histopathological profiles in the rat cornea after alkali injury over a 21-day period.<br />Methods: Alkali injury was induced in one eye of male Lewis rats. Corneal opacity and corneal neovascularization were assessed daily. Real-time qRT-PCR analysis and immunohistochemical staining were conducted to examine inflammation, neovascularization, and fibrosis.<br />Results: We found that within 2 hours of chemical exposure, corneal opacification rapidly developed with an acute increase in various cytokine expressions, while several cytokines demonstrated a secondary peak by day 7. Early neutrophil infiltration peaked at day 1 post-injury while macrophage infiltration peaked at day 7. Throughout the time course of the study, corneal opacity persisted and neovascularization, lymphangiogenesis, and fibrosis progressed.<br />Conclusions: This study highlights the molecular, clinical, and histopathological changes throughout the progression of alkali injury in the rat cornea. These profiles will assist in the development of new strategies and therapies for ocular alkali injury.
- Subjects :
- Animals
Burns, Chemical genetics
Burns, Chemical immunology
Cornea drug effects
Cornea pathology
Corneal Neovascularization chemically induced
Corneal Neovascularization genetics
Corneal Neovascularization immunology
Corneal Opacity chemically induced
Corneal Opacity genetics
Corneal Opacity immunology
Cytokines genetics
Enzyme-Linked Immunosorbent Assay
Eye Burns genetics
Eye Burns immunology
Fibrosis
Gene Expression physiology
Inflammation pathology
Lymphangiogenesis immunology
Macrophages immunology
Male
Neutrophils immunology
RNA, Messenger genetics
Rats
Rats, Inbred Lew
Real-Time Polymerase Chain Reaction
Sodium Hydroxide
Wound Healing
Burns, Chemical pathology
Corneal Neovascularization pathology
Corneal Opacity pathology
Disease Models, Animal
Eye Burns chemically induced
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2202
- Volume :
- 42
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Current eye research
- Publication Type :
- Academic Journal
- Accession number :
- 28636415
- Full Text :
- https://doi.org/10.1080/02713683.2017.1317817