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TP73-AS1 promotes breast cancer cell proliferation through miR-200a-mediated TFAM inhibition.
- Source :
-
Journal of cellular biochemistry [J Cell Biochem] 2018 Jan; Vol. 119 (1), pp. 680-690. Date of Electronic Publication: 2017 Oct 05. - Publication Year :
- 2018
-
Abstract
- P73 antisense RNA 1T (TP73-AS1 or PDAM) is a long non-coding RNA, which can regulate apoptosis through regulation of p53 signaling-related anti-apoptotic genes. An abnormal change of TP73-AS1 expression was noticed in cancers. The effects of TP73-AS1 in breast cancer (BC) growth and the underlying mechanism remain unclear so far. In the present study, the effect of TP73-AS1 in BC cell lines and clinical tumor samples was detected so as to reveal its role and function. In the present study, TP73-AS1 was specifically upregulated in BC tissues and BC cell lines and was correlated to a poorer prognosis in patients with BC. TP73-AS1 knocking down suppressed human BC cell proliferation in vitro through regulation of TFAM. In our previous study, we demonstrated that miR-200a inhibits BC cell proliferation through targeting TFAM; here we revealed that TP73-AS1 could regulate miR-200a through direct targeting. Moreover, TP73-AS1 might compete with TFAM for miR-200a binding thus to promote TFAM expression. Data from the present study revealed that TP73-AS1 promoted BC cell proliferation through acting as a competing endogenous RNA (ceRNA) by sponging miR-200a. In conclusion, we regarded TP73-AS1 as an oncogenic lncRNA promoting BC cell proliferation and a potential target for human BC treatment.<br /> (© 2017 Wiley Periodicals, Inc.)
- Subjects :
- 3' Untranslated Regions
Adult
Cell Line, Tumor
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic
Humans
MCF-7 Cells
Middle Aged
Prognosis
Survival Analysis
Up-Regulation
Young Adult
Breast Neoplasms genetics
DNA-Binding Proteins genetics
MicroRNAs genetics
Mitochondrial Proteins genetics
RNA, Long Noncoding genetics
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4644
- Volume :
- 119
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28639399
- Full Text :
- https://doi.org/10.1002/jcb.26231