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Magnetic nanogels as dual triggered anticancer drug delivery: Toxicity evaluation on isolated rat liver mitochondria.
- Source :
-
Toxicology letters [Toxicol Lett] 2017 Aug 15; Vol. 278, pp. 18-29. Date of Electronic Publication: 2017 Jun 19. - Publication Year :
- 2017
-
Abstract
- This study aimed to evaluate bio-safety of magnetic chitosan nanogels as dual triggered drug carrier for doxorubicin through analysis of mitochondrial function. In the present study, chitosan/TPP nanogels containing magnetite nanoparticles (NPs) were prepared according to the ionotropic gelation method as novel pH-sensitive magnetic nanogels. The NPs showed outstanding entrapment efficiency for doxorubicin (76.6%) with a sustained and high extent of drug release in the acidic media (pH=5-7) compared to the neutral media. Various mitochondrial functional parameters including complex II activity, MDA amount, GSH level, membrane potential collapse, swelling, apoptosis and release of cytochrome c were used to investigate the bio-safety of the nanogels. The findings revealed that the extent of mitochondrial dysfunction of doxorubicin were in the order of free doxorubicin>doxorubicin loaded magnetic nanogels=>doxorubicin loaded Nanogels. The results also revealed that the nanogels and the magnetite nanogels seem to possess promising capability as a safe carrier in comparison of the toxic potential effect of free doxorubicin.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Antineoplastic Agents administration & dosage
Antineoplastic Agents chemistry
Apoptosis drug effects
Chitosan chemistry
Cytochromes c metabolism
Dose-Response Relationship, Drug
Doxorubicin administration & dosage
Doxorubicin chemistry
Drug Compounding
Drug Liberation
Glutathione metabolism
Kinetics
Lipid Peroxidation drug effects
Liver metabolism
Liver pathology
Magnetite Nanoparticles chemistry
Malondialdehyde metabolism
Membrane Potential, Mitochondrial drug effects
Microscopy, Electron, Scanning
Mitochondria, Liver metabolism
Mitochondria, Liver pathology
Mitochondrial Swelling drug effects
Risk Assessment
Solubility
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Succinate Dehydrogenase metabolism
Thermogravimetry
Antineoplastic Agents toxicity
Chitosan toxicity
Doxorubicin toxicity
Drug Carriers
Liver drug effects
Magnetics methods
Magnetite Nanoparticles toxicity
Mitochondria, Liver drug effects
Nanomedicine methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 278
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 28642010
- Full Text :
- https://doi.org/10.1016/j.toxlet.2017.06.004