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Yeast RNA-Binding Protein Nab3 Regulates Genes Involved in Nitrogen Metabolism.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2017 Aug 28; Vol. 37 (18). Date of Electronic Publication: 2017 Aug 28 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion.<br /> (Copyright © 2017 American Society for Microbiology.)
- Subjects :
- Catabolite Repression genetics
Codon, Nonsense genetics
Glutamate Plasma Membrane Transport Proteins genetics
Glutamate-Ammonia Ligase antagonists & inhibitors
Glutamate-Ammonia Ligase metabolism
IMP Dehydrogenase biosynthesis
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins biosynthesis
Nitrogen metabolism
Nuclear Proteins metabolism
RNA Polymerase II genetics
RNA, Messenger genetics
RNA, Small Nucleolar genetics
RNA-Binding Proteins metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins metabolism
Transcription Termination, Genetic physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5549
- Volume :
- 37
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 28674185
- Full Text :
- https://doi.org/10.1128/MCB.00154-17