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Chitosan promotes immune responses, ameliorating total mature white blood cell numbers, but increases glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, and ameliorates lactate dehydrogenase levels in leukemia mice in vivo.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2017 Sep; Vol. 16 (3), pp. 2483-2490. Date of Electronic Publication: 2017 Jul 05. - Publication Year :
- 2017
-
Abstract
- The aim of the present study was to investigate the effect of chitosan (a naturally derived polymer) on the immune responses and glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactate dehydrogenase (LDH) levels in WEHI‑3 cell‑generated leukemia mice. Mice were divided into control, WEHI‑3 control, acetic acid (vehicle)‑treated, and 5 and 20 mg/kg chitosan‑treated groups. Mice were subsequently weighed, blood was collected, and liver and spleen samples were isolated and weighed. Blood samples were measured for cell markers, the spleen underwent phagocytosis and natural killer (NK) cell activity examination, and cell proliferation was analyzed by flow cytometry. Chitosan did not significantly affect the weights of body, liver and spleen at 5 and 20 mg/kg treatment. Chitosan increased the percentage of CD3 (T cells marker), decreased the levels of CD19 (B‑cell marker) and CD11b at 5 mg/kg treatment, and decreased the levels of Mac‑3 at 5 and 20 mg/kg treatment. Chitosan significantly increased macrophage phagocytosis of PBMCs, but did not significantly affect macrophage phagocytosis in the peritoneal cavity. Chitosan treatment did not significantly affect the cytotoxic activity of NK cells, and also did not affect T- and B-cell proliferation. Chitosan significantly increased total white blood cell numbers, and GOT and GPT activities were both significantly increased. However, chitosan did not significantly affect LDH activity in leukemia mice. Chitosan may aid in future studies on improving immune responses in the treatment of leukemia.
- Subjects :
- Adjuvants, Immunologic pharmacology
Alanine Transaminase immunology
Animals
Aspartate Aminotransferases immunology
Cell Line, Tumor
Chitosan pharmacology
Cytotoxicity, Immunologic drug effects
Killer Cells, Natural drug effects
Killer Cells, Natural immunology
L-Lactate Dehydrogenase immunology
Leukemia blood
Leukemia immunology
Leukocyte Count
Lymphocyte Activation drug effects
Male
Mice
Mice, Inbred BALB C
Phagocytosis drug effects
Adjuvants, Immunologic therapeutic use
Alanine Transaminase blood
Aspartate Aminotransferases blood
Chitosan therapeutic use
Immunity, Cellular drug effects
L-Lactate Dehydrogenase blood
Leukemia drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 16
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 28677783
- Full Text :
- https://doi.org/10.3892/mmr.2017.6923