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Extraction of Lactobacillus acidophilus CICC 6074 S-Layer Proteins and Their Ability to Inhibit Enteropathogenic Escherichia coli.
- Source :
-
Current microbiology [Curr Microbiol] 2017 Oct; Vol. 74 (10), pp. 1123-1129. Date of Electronic Publication: 2017 Jul 07. - Publication Year :
- 2017
-
Abstract
- An adhesion-related protein of Lactobacillus acidophilus strain CICC 6074 involved in binding to Caco-2 cells and inhibiting Enteropathogenic Escherichia coli (EPEC) was isolated and characterized. The S-layer protein was extracted with 5M LiCl and the active fraction purified by gel filtration (G-75). The S-layer protein was visualized by SDS-PAGE and characterized by estimating the relative molecular weight using mass spectra. The inhibitory effect of L. acidophilus and its S-layer proteins on the ability of EPEC to adhere to cells was explored by using a Caco-2 cell model. The results suggest that the S-layer proteins of L. acidophilus are adhesive in nature and are involved in the competitive exclusion of EPEC from Caco-2 cells.
- Subjects :
- Amino Acid Sequence
Bacterial Adhesion
Caco-2 Cells
Humans
Membrane Glycoproteins chemistry
Membrane Glycoproteins isolation & purification
Membrane Glycoproteins pharmacology
Microbial Viability
Probiotics
Antibiosis
Enteropathogenic Escherichia coli physiology
Lactobacillus acidophilus physiology
Membrane Glycoproteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0991
- Volume :
- 74
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 28687945
- Full Text :
- https://doi.org/10.1007/s00284-017-1291-1