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Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy.
- Source :
-
Biophysical journal [Biophys J] 2017 Sep 19; Vol. 113 (6), pp. 1321-1330. Date of Electronic Publication: 2017 Jul 19. - Publication Year :
- 2017
-
Abstract
- The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles, show the high potential of these dyes for probing nanoscale membrane heterogeneity.<br /> (Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
CHO Cells
Cell Membrane
Cricetulus
Cytoplasmic Vesicles
HEK293 Cells
HIV
Humans
Molecular Structure
Phosphatidylethanolamines chemistry
Spectrum Analysis
Virion chemistry
Benzoxazines chemistry
Fluorescent Dyes chemistry
Microscopy methods
Pyridinium Compounds chemistry
Quaternary Ammonium Compounds chemistry
Voltage-Sensitive Dye Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1542-0086
- Volume :
- 113
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biophysical journal
- Publication Type :
- Academic Journal
- Accession number :
- 28734477
- Full Text :
- https://doi.org/10.1016/j.bpj.2017.06.050