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Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy.

Authors :
Sezgin E
Schneider F
Zilles V
Urbančič I
Garcia E
Waithe D
Klymchenko AS
Eggeling C
Source :
Biophysical journal [Biophys J] 2017 Sep 19; Vol. 113 (6), pp. 1321-1330. Date of Electronic Publication: 2017 Jul 19.
Publication Year :
2017

Abstract

The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles, show the high potential of these dyes for probing nanoscale membrane heterogeneity.<br /> (Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1542-0086
Volume :
113
Issue :
6
Database :
MEDLINE
Journal :
Biophysical journal
Publication Type :
Academic Journal
Accession number :
28734477
Full Text :
https://doi.org/10.1016/j.bpj.2017.06.050