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Buddleja thyrsoides Lam. crude extract presents antinociceptive effect on an arthritic pain model in mice.
- Source :
-
The Biochemical journal [Biochem J] 2017 Aug 17; Vol. 474 (17), pp. 2993-3010. Date of Electronic Publication: 2017 Aug 17. - Publication Year :
- 2017
-
Abstract
- Arthritis is a chronic inflammatory disease which reduces the life quality of affected individuals. Therapeutic tools used for treating inflammatory pain are associated with several undesirable effects. Buddleja thyrsoides Lam., known as 'Barbasco' or 'Cambara', is mostly used in several disorders and possesses antirheumatic, anti-inflammatory, and analgesic properties. Here, we investigated the antinociceptive and anti-inflammatory effects of the B. thyrsoides crude extract applied orally and topically in acute pain models and an arthritic pain model induced by complete Freund's adjuvant (CFA) paw injection in male mice (25-30 g). The high-performance liquid chromatography (HPLC) of the B. thyrsoides extract crude revealed the presence of the lupeol, stigmasterol, and β-sitosterol. The stability study of the B. thyrsoides gel did not show relevant changes at low temperatures. The oral treatment with the B. thrysoides extract prevented the capsaicin-induced spontaneous nociception and the acetic acid-induced abdominal writhing, but did not alter the thermal threshold in the tail immersion test. The B. thyrsoides antinociceptive effect was not reversed by naloxone in the capsaicin test. The B. thyrsoides oral or topical treatment reversed the CFA-induced mechanical allodynia and thermal hyperalgesia with maximum inhibition ( I <subscript>max</subscript> ) of 69 ± 6 and 68 ± 5% as well as 78 ± 15 and 87 ± 12%, respectively. Moreover, the topical but not oral treatment inhibited the CFA-induced cell infiltration, but did not reduce the paw edema significantly. The oral treatment with B. thyrsoides did not cause adverse effects. These findings suggest that the oral or topical treatment with B. thyrsoides presents antinociceptive actions in an arthritic pain model without causing adverse effects.<br /> (© 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
- Subjects :
- Acute Pain drug therapy
Administration, Cutaneous
Administration, Oral
Analgesics, Non-Narcotic administration & dosage
Analgesics, Non-Narcotic adverse effects
Analgesics, Non-Narcotic chemistry
Animals
Anti-Inflammatory Agents, Non-Steroidal administration & dosage
Anti-Inflammatory Agents, Non-Steroidal adverse effects
Anti-Inflammatory Agents, Non-Steroidal chemistry
Brazil
Buddleja growth & development
Drug Stability
Drug Storage
Ethnopharmacology
Gels
Hot Temperature adverse effects
Male
Mice
Pentacyclic Triterpenes administration & dosage
Pentacyclic Triterpenes adverse effects
Pentacyclic Triterpenes analysis
Pentacyclic Triterpenes therapeutic use
Plant Extracts administration & dosage
Plant Extracts adverse effects
Plant Extracts chemistry
Plant Leaves growth & development
Sitosterols administration & dosage
Sitosterols adverse effects
Sitosterols analysis
Sitosterols therapeutic use
Stigmasterol administration & dosage
Stigmasterol adverse effects
Stigmasterol analysis
Stigmasterol therapeutic use
Viscosity
Analgesics, Non-Narcotic therapeutic use
Anti-Inflammatory Agents, Non-Steroidal therapeutic use
Arthritis, Experimental drug therapy
Buddleja chemistry
Plant Extracts therapeutic use
Plant Leaves chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1470-8728
- Volume :
- 474
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- The Biochemical journal
- Publication Type :
- Academic Journal
- Accession number :
- 28739601
- Full Text :
- https://doi.org/10.1042/BCJ20170008